| 基于线粒体控制区的中国南海海域卵形鲳鲹遗传多样性 |
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| 引用本文： | 吕金磊,章群,杨喜书,宫亚运,曹艳.基于线粒体控制区的中国南海海域卵形鲳鲹遗传多样性[J].海洋渔业,2017,39(3). |
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| 作者姓名： | 吕金磊 章群 杨喜书 宫亚运 曹艳 |
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| 作者单位： | 暨南大学水生生物研究中心,教育部热带亚热带水生态工程研究中心,广州510632 |
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| 基金项目： | 国家自然科学基金项目，中央高校基本科研业务费专项资金项目 |
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| 摘 要： | 为探讨中国南海重要经济鱼类卵形鲳鲹(Trachinotus ovatus)的遗传多样性,测定了广东闸坡、乌石、安铺和广西东兴以及海南新盈等5个地理群体97 ind样品的线粒体控制区5'端359 bp序列,发现47个变异位点,32个单倍型,总体呈现高单倍型多样性(h=0.951)和高核苷酸(π=0.020 9)多样性的特点。在邻接树和单倍型网络图中出现2个分化显著但不存在明显地理聚群的分支,推测二者的分化时间约为60~18万年前(中更新世),可能是中更新世冰期海平面下降形成边缘海而导致隔离,间冰期海平面上升后出现二次接触。不同地理群体间的遗传分化不显著(Fst=-0.022 4~0.045 3),AMOVA分析也显示97%以上的遗传变异来源于群体内个体间。卵形鲳鲹2个谱系及总体的核苷酸错配图呈现多峰,中性检验均为负值不显著(P0.05),表明都未经历过大规模的种群扩张,处于相对稳定的状态。
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| 关 键 词： | 卵形鲳鲹 中国南海 线粒体控制区 遗传多样性 |
Genetic diversity of Trachinotus ovatus in the South China Sea inferred from Mitochondrial DNA control region sequences |
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| LV Jin-lei,ZHANG Qun,YANG Xi-shu,GONG Ya-yun,CAO Yan.Genetic diversity of Trachinotus ovatus in the South China Sea inferred from Mitochondrial DNA control region sequences[J].Marine Fisheries,2017,39(3). |
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| Authors: | LV Jin-lei ZHANG Qun YANG Xi-shu GONG Ya-yun CAO Yan |
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| Abstract: | Due to the delicious taste,high nutritional value and increasing market demand,Trachinotus ovatus has become one of the most economically important maricultured fish in the South China Sea.With the dwindling wild resources,the development of large-scale cultivation and the degraded germplasm of cultivars etc.,the accurate definition of population structure should be assessed for better management and sustainable exploitation of T.Ovatus resource in the South China Sea.In present study,359 bp mtDNA control region sequences of 97 individuals of T.Ovatus from Zhapo,Anpu and Wushi in Guangdong Province,Xinying in Hainan Province and Dongxing in Guangxi Province were analyzed to study the genetic diversity of this species in the South China Sea.A total of 47 polymorphic loci were found,of which 39 sites were parsimonyinformative and 32 haplotypes were defined.The resultant haplotype diversity (h) and nucleotide diversity (πr) were 0.951 ± 0.008 and 0.020 9 ± 0.000 8 respectively,indicating the high level of haplotype diversity and nucleotide diversity of T.Ovatus populations in north of the South China Sea.In Neighborjoining tree and TCS network,two lineages were detected,and individuals from various geographical sites scattered in both lineages,thus no geographical cluster was found.The deduced differentiation time was approximately 600 ～180 ka BP.Despite the presence of two co-existing lineages,the absence of genetic heterogeneity among the 5 sampling sites was noted,as the genetic fixation was low and not significant(Fst =-0.022 4 ～ 0.045 3,P ＞ 0.05),and the genetic distances were small (0.020-0.023).The analysis of molecular variance (AMOVA) also revealed that most of genetic variation resided within populations (＞ 97％).This pattern of harboring obvious lineage structure but no geographic structure might have been shaped by glaciation cycles in Pleistocene.During the ice age,the closure of marginal seas caused by deeply lowered sea level might have prevented gene flow among seperate seas,resulting in lineages differentiation.In interglacial stage,the rising of sea level might have reconnected marginal seas,promoting the secondary contact of once-isolated lineages,as the warm current of the South China Sea and the China Coastal current might have carried away fish eggs and larvae of T.Ovatus,and the formation of feeding and spawning schools also promoted the gene flow.Both nucleotide mismatch distribution and neutrality tests revealed that the lineage A,lineage B,and all populations as a whole in the South China Sea hadn't experienced population expansion.In conclusion,although the genetic diversity of T.Ovatus in the South China Sea is relatively high yet,in the context of over exploitation of wild resources,degraded the germplast of cultivars,and the potential negative effects of breeding escape on their genetic diversity,proper management and sustainable exploitation of this species are urgently deserved.In further study,more populations across the species' range should be analyzed to determine the potential population genetic structure and demographic history with both mitochondrial and nuclear information. |
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| Keywords: | Trachinotus ovatus South China Sea mtDNA control region genetic diversity |
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