Co-migrating and shared antigens of selectedPasteurella haemolytica untypable strains |
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| Authors: | K R Simons R J Morton D A Mosier R W Fulton A W Confer |
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| Institution: | (1) Department of Veterinary Parasitology, Microbiology and Public Health, College of Veterinary Medicine, Oklahoma State University, 74078 Stillwater, OK, USA;(2) Department of Veterinary Pathology, College of Veterinary Medicine, Oklahoma State University, 74078 Stillwater, OK, USA;(3) Present address: Department of Pathology, Veterinary Clinical Sciences Building, Kansas State University, 66506 Manhattan, KS, USA |
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| Abstract: | Bacterial cell envelope preparations from eight untypable strains ofPasteurella haemolytica were compared by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and immunoblotting with rabbit antisera prepared against the eight untypable strains (one untypable strain per rabbit) and with cattle antisera prepared againstP. haemolytica serotypes 1, 2, 5, 6, 9 and against one heterologous untypable strain. Numerous comigrating and shared antigens were recognized by the eight rabbit antisera and theP. haemolytica serotype cattle antisera. Comigrating antigens at 43 and 30 kilodaltons (kDa) were recognized by all eight rabbit antisera. Shared antigens, detected by all eight rabbit antisera when reacting againstP. haemolytica serotype 1, were recognized at 43, 32, 30, 20 and 15 kDa. |
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| Keywords: | antigen electrophoresis immunoblotting Pasteurella haemolytica untypable |
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