| 白杂1号种子纯度的RAPD鉴定技术研究 |
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| 引用本文： | 刘静,董振生,李红兵.白杂1号种子纯度的RAPD鉴定技术研究[J].西北农业学报,2007,16(1):131-135. |
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| 作者姓名： | 刘静 董振生 李红兵 |
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| 作者单位： | 1. 西北农林科技大学动物科技学院,陕西杨凌,712100
2. 西北农林科技大学农学院,陕西杨凌,712100 |
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| 摘 要： | 以白菜型油菜新品种“白杂1号”父母本及F1代种子为研究材料,用238个10-mer随机引物对它们进行了RAPD分析鉴定,结果显示共有68个随机引物出现多态性扩增,DNA片断大小在0.17~1.8 kb之间。经多次重复验证,引物S24、S83和S104条带稳定,并且杂交种条带为双亲互补型;引物S176为偏父型,在母本中扩增出350 bp特征带,而其在父本中呈阴性。选取S104(GGAAGTCGCC)对两个亲本及F1建立指纹图谱,共检测出6条强带,其中380 bp是母本特征带,1 600 bp是父本特征带。人为掺杂构建“白杂1号”F1代测试群体,用S104对其进行了纯度检测,结果与预期结果基本一致。对RAPD方法在鉴定种子纯度中的有关双引物混合扩增和条带判断取舍问题进行了讨论。
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| 关 键 词： | 白菜型油菜 种子纯度 |
| 文章编号： | 1004-1389（2007）01-0131-05 |
| 收稿时间： | 2006-04-12 |
| 修稿时间： | 2006-07-22 |
Identification of Genetic Purity of the Hybrid "Baiza No.1" with RAPD in Brassica compestris L. |
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| LIU Jing,DONG Zhen-sheng and LI Hong-bing.Identification of Genetic Purity of the Hybrid "Baiza No.1" with RAPD in Brassica compestris L.[J].Acta Agriculturae Boreali-occidentalis Sinica,2007,16(1):131-135. |
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| Authors: | LIU Jing DONG Zhen-sheng and LI Hong-bing |
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| Institution: | College of Animal Science and Technology, Northwest A & F University, Yangling Shaanxi 712100, China;College of Agronomy, Northwest A & F University, Yangling Shaanxi 712100, China;College of Agronomy, Northwest A & F University, Yangling Shaanxi 712100, China |
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| Abstract: | Both hybrid rapeseed "Baiza No.1" and its parents were analyzed by RAPD markers in this study,the results showed that 68 primers,which were selected from 238 10-mer RAPD primers,could generated polymorphic products ranging from 170~1,800 base pairs(bp).By analyzing of their fingerprints,S24,S83 and S104 could produce both maternal-specific and paternal-specific DNA bands and the reproducibility was steady after repeating the tests,while S176 only could produce specific bands to the mother.By comparison,S104 was the best to be used in identification of the purity in F1.It could produce 6 bright bands: one is the fragment at 380bp which was unique to the male line,another was the fragment at 1600bp which was unique to the female line and the two fragments were complementary in the F1 generation.Result based on RAPD markers by primer S104 showed that 23 rapeseeds were not real hybrids in the self-established F1 population of "Baiza No.1" which was composed of 10 maternal rapeseeds,10 paternal rapeseeds and 80 hybrids.The above accorded with expected purity result.In addition,double-primer method of PCR and the option standard of bands of identification of the purity were discussed in this report. |
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| Keywords: | RAPD |
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