Ploidy assessment of porcine haploid and diploid parthenogenetic embryos by fluorescent in situ hybridization detecting a chromosome 1-specific sequence, Sus scrofa Mc1 satellite DNA |
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| Authors: | Sembon Shoichiro Fuchimoto Dai-ichiro Iwamoto Masaki Suzuki Shun-ichi Onishi Akira |
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| Institution: | Transgenic Animal Research Center, National Institute of Agrobiological Sciences, Ibaraki, Japan. senbon@affrc.go.jp |
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| Abstract: | The aim of the present study was to examine the feasibility of fluorescent in situ hybridization (FISH) for detecting a chromosome 1-specific sequence as a means of assessing the ploidy of porcine parthenotes. In vitro-matured oocytes with the first polar body (PB) were electrically activated; some were treated with cytochalasin B to prevent second PB extrusion (1PB embryos), and the others extruded the second PB (2PB embryos). At the 2-cell stage, one and two FISH signals were detected in each nucleus of 2PB and 1PB embryos, respectively. Almost all cells of blastocysts derived from 1PB embryos retained two signals. In contrast, cells of blastocysts derived from 2PB embryos had two signals. These data demonstrate that FISH analysis allows precise ploidy assessment of porcine parthenogenetic embryos, hence providing a practical means of detecting ploidy transition during parthenogenetic embryogenesis. |
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