| 白花泡桐优树试管嫁接幼化及组培快繁技术研究 |
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| 引用本文： | 邓建军,李芳东,乔杰,刘昌勇,夏辛,黄琳.白花泡桐优树试管嫁接幼化及组培快繁技术研究[J].林业科学研究,2011,24(5):646-650. |
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| 作者姓名： | 邓建军 李芳东 乔杰 刘昌勇 夏辛 黄琳 |
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| 作者单位： | 国家林业局泡桐研究开发中心,郑州,450003 |
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| 基金项目： | 林业公益性行业科研专项"泡桐基因库与育种群体建立技术研究"(200804021) |
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| 摘 要： | 以白花泡桐优树‘白优2号’为试验材料,通过组织培养和试管嫁接方法,对白花泡桐优树材料的幼化技术进行了研究.结果表明:外植体初代培养萌发的嫩芽为最适合的接穗;‘建始桐3号’为试管嫁接较合适的砧木;采用劈接进行;MS+ NAA0.3 mg·L-1+蔗糖30 g·L-1为试管嫁接培养基;继代增殖和生根培养基分别为1/2MS ...
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| 关 键 词： | 白花泡桐 优树 组培快繁 试管嫁接 幼化 |
| 收稿时间： | 2010/12/20 0:00:00 |
Study on Grafted Tissue Rejuvenation Technology and Rapid Propagation
of Paulownia fortunei Superior Trees |
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| DENG Jian-jun,LI Fang-dong,QIAO Jie,LIU Chang-yong,XIA Xin and HUANG Lin.Study on Grafted Tissue Rejuvenation Technology and Rapid Propagation
of Paulownia fortunei Superior Trees[J].Forest Research,2011,24(5):646-650. |
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| Authors: | DENG Jian-jun LI Fang-dong QIAO Jie LIU Chang-yong XIA Xin and HUANG Lin |
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| Institution: | DENG Jian-jun,LI Fang-dong,QIAO Jie,LIU Chang-yong,XIA Xin,HUANG Lin(Paulownia Research & Development Center of the State Forestry Administration,Zhengzhou 450003,He'nan,China) |
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| Abstract: | Paulownia fortunei superior trees "Baiyou 2" were used as trial materials to explore the rejuvenation technology of P. fortunei superior tree by tissue culture and in vitro grafting. The results are as follows: (1) The sprouting of explant is the most suitable scion; (2) The Paulownia variety "Jianshi 3" is a good rootstock; (3) Split grafting is a suitable; (4) 1/2 MS+6-BA 4.0 mg·L-1+NAA 0.3 mg·L-1 is the most appropriate medium for in vitro grafting, 1/2 MS+6-BA 6.0 mg·L-1+NAA 0.3 mg·L-1+Sucrose 20 g·L-1 is the most appropriate for multiplication and 1/2 MS+NAA 0.1 mg·L-1 is the most appropriate for rooting. The old Paulownia superior tree material was rejuvenated successfully and the rapid propagation was realized. |
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| Keywords: | Paulownia fortunei superior tree tissue culture and rapid propagation in vitro grafting rejuvenation |
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