| 不同菌落形态的链霉菌对产谷氨酰胺转胺酶的影响 |
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| 引用本文： | 宋敏,曹娟,张颖颖,高红亮,范西强,鲁心安.不同菌落形态的链霉菌对产谷氨酰胺转胺酶的影响[J].广西农业生物科学,2008,27(4):435-438. |
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| 作者姓名： | 宋敏 曹娟 张颖颖 高红亮 范西强 鲁心安 |
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| 作者单位： | 华东师范大学,生命科学学院,上海200062 |
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| 基金项目： | 国家自然科学基金项目(30600079) |
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| 摘 要： | 通过对链霉菌STK-203进行自然选育，挑选出5种不同菌落形态的单菌落。摇瓶发酵培养48h后检测发酵液中的谷氨酰胺转胺酶的活性、残余氨基氮含量、残余甘油含量、菌种生物量等4种生理指标。结果表明，编号为Ⅰ即馒头型的单菌落的酶活最高，高达5．84U／mL，其残余甘油含量为0．071g／L，残余氨基氮含量为1．48g／L，菌体浓度为36．01％。根据菌落形态和产酶的相关性，为产谷氨酰胺转胺酶的链霉菌STK-203菌株的选育和生产提供了一种简便有效的方法。
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| 关 键 词： | 链霉菌STK-203 谷氨酰胺转胺酶 菌落形态 |
Effect of different colonial morphologies on Transglutaminase production of Streptomyces |
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| SONG Min,CAO Juan,ZHANG Ying-ying,GAO Hong-liang,FAN Xi-qiang,LU Xin-an.Effect of different colonial morphologies on Transglutaminase production of Streptomyces[J].Journal of Guangxi Agricultural and Biological Science,2008,27(4):435-438. |
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| Authors: | SONG Min CAO Juan ZHANG Ying-ying GAO Hong-liang FAN Xi-qiang LU Xin-an |
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| Institution: | (School of Life Science, East China Normal University, Shanghai 200062, China) |
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| Abstract: | Five colonies of different morphologies of Streptomyces STK-203 were obtained by natural selection.Microbial transglutaminase(MTG)activity,residual nitrogen content,residual glycerine content and cell concentration by shaking-flask fermentation trials were measured.Results showed that the MTG activity of NO.1 of morphology was the highest,up to 5.84 U/mL.Its residual glycerine content was 0.071 g/L,and its residual nitrogen content was 1.48 g/L,and its cell concentration was 36.01%.The relationship of MTG a... |
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| Keywords: | Streptomyces STK-203 Transglutaminase colonial morphology |
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