| 猪繁殖与呼吸综合征病毒ORF5a/ORF5重叠区的分离 |
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| 引用本文： | 刘闰夏,蔺涛,田德斌,韦祖樟,孙利厂,陆嘉琦,袁世山,童光志.猪繁殖与呼吸综合征病毒ORF5a/ORF5重叠区的分离[J].中国动物传染病学报,2012(2):29-35. |
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| 作者姓名： | 刘闰夏 蔺涛 田德斌 韦祖樟 孙利厂 陆嘉琦 袁世山 童光志 |
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| 作者单位： | 中国农业科学院上海兽医研究所;南京农业大学动物医学院 |
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| 基金项目： | 国家自然科学基金面上项目(30972204) |
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| 摘 要： | 以两个不同基因型的猪繁殖与呼吸综合征病毒(Porcine reproductive and respiratory syndrome virus,PRRSV)感染性克隆为平台进行反向遗传操作,利用SOE-PCR将I型PRRSV的ORF2～5a替换进II型PRRSV的相应区域,旨在将I型PRRSV的5a从ORF5中独立出来进而研究5a蛋白的功能,同时探讨两型PRRSV的结构蛋白结构与功能关系。研究结果表明,I型PRRSV的ORF2～5a序列能稳定存在于嵌合病毒基因组内,表达的相应蛋白能在II型PRRSV中发挥同样功能。本研究首次将PRRSV的ORF5a序列从ORF5中独立出来,为进一步研究5a蛋白的结构与功能提供了理论依据和基础材料。
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| 关 键 词： | 猪繁殖与呼吸综合征病毒 嵌合 重叠序列 5a蛋白 |
THE SEPERATION OF ORF5A/ORF5 OVERLAPPING GENES OF PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRVS |
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| LIU Run-xia,LIN Tao,TIAN De-bin,WEI Zu-zhang,SUN Li-chang,LU Jia-qi,YUAN Shi-shan,TONG Guang-zhi.THE SEPERATION OF ORF5A/ORF5 OVERLAPPING GENES OF PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRVS[J].Chinese Journal of Animal Infectious Diseases,2012(2):29-35. |
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| Authors: | LIU Run-xia LIN Tao TIAN De-bin WEI Zu-zhang SUN Li-chang LU Jia-qi YUAN Shi-shan TONG Guang-zhi |
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| Institution: | 1(1.Shanghai Veterinary Research Institute,CAAS,Shanghai 200241,China;2.College of Veterinary Medicine, Nanjing Agricultural University,Nanjing 210095,China) |
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| Abstract: | For the first time,a new chimeric Porcine reproductire and respiratory syndrome virus(PRRSV) was constructed by replacing ORF2-5a of the full-length infectious cDNA clone pARRSasc(type 2) with their corresponding counterparts of the clone pSHE(type 1).This study was designed to(1) make ORF5a independent of ORF5;(2) dissertate the structure and function of 5a protein;(3) elucidate the structure-function relationship of inter-genotypic PRRSV structural proteins.The results showed that ORF2-5a of PRRSV type 1 was stable and expressed structural proteins and played full functions in the backbone type 2 virus.The construction of the chimeric PRRSV expressing ORF5a independent of ORF5 has laid foundation to better understand the structure and function of 5a protein. |
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| Keywords: | Porcine reproductive and respiratory syndrome virus(PRRSV) chimeric overlapping genes 5a protein |
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