| 藤茶HPLC指纹图谱 |
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| 引用本文： | 陈帅,郁建平.藤茶HPLC指纹图谱[J].贵州大学学报(农业与生物科学版),2011,30(3):275-279. |
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| 作者姓名： | 陈帅 郁建平 |
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| 作者单位： | 贵州大学生命科学学院,贵州贵阳,550025 |
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| 基金项目： | 贵州省科技厅项目[黔科合县市科技(2009)7012-1] |
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| 摘 要： | 为了提供藤茶药材质量标准的依据,本文采用HPLC法建立了藤茶药材指纹图谱.色谱条件为迪马C 18色谱柱(4.6mm×250mm,5μm),流动相为乙腈-水(0.1％)磷酸,梯度洗脱,流速1.0mL/min,检测波长291 nmm.采用中华人民共和国药典委员会出版的中药色谱指纹图谱相似度评价系统软件(版本:2004A)进...
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| 关 键 词： | 藤茶 显齿蛇葡萄 HPLC 指纹图谱 |
HPLC Fingerprint of Ampelopsis grossedentata |
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| CHEN Shuai,YU Jian-ping.HPLC Fingerprint of Ampelopsis grossedentata[J].Journal of Mountain Agriculture & Biology,2011,30(3):275-279. |
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| Authors: | CHEN Shuai YU Jian-ping |
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| Institution: | CHEN Shuai,YU Jian-ping(College of Life Sciences,Institute of Biochemistry and Nutrition,Guizhou University,Guiyang Guizhou 550025,China) |
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| Abstract: | To provide the basis for quality standards of Ampelopsis grossedentata,the fingerprints of Ampelopsis grossedentata were established by HPLC in the present study.The samples were separated by a Diamonsil C18 column（4.6 mm×250 mm,5 μm） with acetonitrile-0.1%H3PO4 gradient system as mobile phase.The flow rate was 1.0 mL·min-1and the detective wave length for the fingerprinting was at 291 nm.Then the chromatograms were compared with ＂Similarity Evaluation System for Chromatographic Fingerprint of TCM＂ software published by ChPC（Version 2004A）.Therefore,HPLC fingerprint were established with 10 marked common peaks,and the methodology satisfy the requirements of fingerprint technology.The obtained method was stable and reliable with good repeatability,which may be used to establish HPLC fingerprint of Ampelopsis grossedentata（Hand-Mazz） W.T.Wang. |
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| Keywords: | Ampelopsis grossedentata HPLC Fingerprint |
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