| 干酪乳杆菌LC2W胞外多糖的体外免疫活性初步研究 |
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| 引用本文： | 刘翠平,王荫榆,吴正钧.干酪乳杆菌LC2W胞外多糖的体外免疫活性初步研究[J].乳业科学与技术,2011,34(1):6-8. |
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| 作者姓名： | 刘翠平 王荫榆 吴正钧 |
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| 作者单位： | 光明乳业股份有限公司,技术中心,乳业生物技术国家重点实验室,上海,200436 |
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| 基金项目： | 国家973计划(项目编号:2010CB735705); 上海乳业生物工程技术研究中心(项目编号:09DZ2251400) |
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| 摘 要： | 通过细胞毒性试验和细胞增殖试验对干酪乳杆菌LC2W产胞外多糖纯化两组分G1和G2的体外免疫活性进行了初步研究,体外细胞培养试验结果表明,G1和G2均无细胞毒性,在5μg/mL~80μg/mL的浓度范围内,G1与G2对T淋巴细胞增殖的作用差异较大,G1与G2对B淋巴细胞增殖均表现为抑制作用,而且G2的抑制作用比G1强,具有明显的剂量效应,其抑制作用与浓度呈正相关。
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| 关 键 词： | 干酪乳杆菌 胞外多糖 免疫活性 |
Study on Immunomodulating Activity of Exopolysaccharides from Lactobacillus casei LC2W |
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| Liu Cuiping,Wang Yinyu,Wu Zhengjun.Study on Immunomodulating Activity of Exopolysaccharides from Lactobacillus casei LC2W[J].JOURNAL OF DAIRY SCIENCE AND TECHNOLOGY,2011,34(1):6-8. |
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| Authors: | Liu Cuiping Wang Yinyu Wu Zhengjun |
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| Institution: | Liu Cuiping,Wang Yinyu,Wu Zhengjun State Key Laboratory of dairy Biotechnology,Technology Center,Bright Dairy & Food Co.Ltd.,Shanghai 200436,China |
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| Abstract: | Immunocompetence of two components（G1 and G2） of exopolysaccharides（EPS） from Lactobacillus casei LC2W was preliminary studied by cytotoxicity and cell proliferation experiments cultured in vitro.The results showed that G1 and G2 had no cytotoxicity to lymphocytes.With concentration of G1 or G2 in the range of 5 μg/mL~80 μg/mL,the proliferation of T-lymphocytes was different between G1 and G2,but both demonstrated inhibitory activity to the proliferation of B-lymphocytes,and the inhibitory activity of G2 were stronger than those of G1 and positively correlated to the concentration of EPS. |
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| Keywords: | Lactobacillus casei exopolysaccharides immunocompetence |
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