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| 热胁迫对大豆花荚离层细胞HSP70基因表达、能量供应及花荚脱落率的影响 | |
| 引用本文： | 柴国华,吕慧颖,李辉亮,陈建南,聂晶,张利明,朱保葛.热胁迫对大豆花荚离层细胞HSP70基因表达、能量供应及花荚脱落率的影响[J].农业生物技术学报,2006,14(4):574-577. |
| 作者姓名： | 柴国华 吕慧颖 李辉亮 陈建南 聂晶 张利明 朱保葛 |
| 作者单位： | 1. 中国科学院遗传与发育生物学研究所,北京,100101;西北农林科技大学农学院,杨陵,712100 2. 中国科学院遗传与发育生物学研究所,北京,100101 3. 中国科学院遗传与发育生物学研究所,北京,100101;湖南农业大学农学院,长沙,410128 |
| 基金项目： | 国家自然科学基金;国家重点基础研究发展计划(973计划);国家科技攻关计划 |
| 摘 要： | 实验运用Northern blot检测和生理生化分析等技术研究热胁迫对大豆(Glycine max)品种花荚离层细胞的HSP70基因表达、能量供应及花荚脱落的影响。结果显示，热胁迫能够提高所有6个供试大豆品种花荚离层细胞的HSP70基因表达水平、线粒体及其蛋白含量以及细胞色素氧化酶表达量，而不同程度地降低了所有供试品种的花荚脱落率。结果说明，大豆花荚离层细胞HSP70基因的高效表达可能是通过有效地改善花荚离层细胞的能量供应，从而达到抑制花荚脱落的目的。关键词: 大豆；热胁迫；HSP70基因；线粒体；细胞色素氧化酶；花荚脱落率 |
| 关 键 词： | 大豆 热胁迫 HSP70基因 线粒体 细胞色素氧化酶 花荚脱落率 |
| 文章编号： | 1006-1304（2006）04-0574-04 |
| 收稿时间： | 2005-07-04 |
| 修稿时间： | 2005-11-10 |
Effects of Heat Stress on HSP70 Gene Expression and Energy Supply in Abscission Zone of Flowers-pods, and Abscission Rate of Flowers-pods in Soybean Cultivars |
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| CHAI Guo-hua,LU Hui-ying,LI Hui-liang,CHEN Jian-nan,NIE Jing,ZHANG Li-ming,ZHU Bao-ge.Effects of Heat Stress on HSP70 Gene Expression and Energy Supply in Abscission Zone of Flowers-pods, and Abscission Rate of Flowers-pods in Soybean Cultivars[J].Journal of Agricultural Biotechnology,2006,14(4):574-577. | |
| Authors: | CHAI Guo-hua LU Hui-ying LI Hui-liang CHEN Jian-nan NIE Jing ZHANG Li-ming ZHU Bao-ge |
| Institution: | 1 .Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101,China; 2. College of Agriculture, Northwest Agriculture and Forestry University, Yangling 712100,China; 3. College of Agriculture, Hunan Agricultural University, Changsha 410128,China |
| Abstract: | The effects of heat stress(HS) on HSP70 gene expression, energy supply in abscission zone of flower-pod and the abscission rate of flowers-pods in soybean (Glycine max) cultivars were analyzed by Northern blot and physiological and biochemical assay. The results showed that HS could lead to the increases of HSP70 gene expression level, mitochondria and their protein contents, and cytochrome oxidase content in abscission zone of flowers-pods, but the decrease of the abscission rate of flowers-pods in all six soybean cultivars comparison with their control groups with normal temperature treatment. The results suggested that the overexpression of HSP70 gene caused the increases of mitochondria and their protein contents and cytochrome oxidase content, which improved enhancement of energy supply in abscission zone of flowers-pods so that this inhibited the abscission of flowers-pods of soybean plants. This will be of importance for reducing abscission rate of flowers-pods and increasing seed yield in soybean by genetic engineering approach. |
| Keywords: | soybean heat stress HSP70 gene mitochondria cytochrome oxidase abscission rate of flowers-pods |
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