| 沟叶结缕草高温胁迫的基因差异表达及其作用机制 |
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| 引用本文： | 黄锦文,吴文祥,陈冬梅,郑红艳,林文雄.沟叶结缕草高温胁迫的基因差异表达及其作用机制[J].草地学报,2009,17(4):435-439. |
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| 作者姓名： | 黄锦文 吴文祥 陈冬梅 郑红艳 林文雄 |
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| 作者单位： | 福建农林大学农业生态研究所, 福州, 350002 |
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| 基金项目： | 福建省生态学重点学科资助 |
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| 摘 要： | 为了从基因表达水平研究沟叶结缕草(Zoysia matrella)高温耐热性的分子机制,利用SSH方法构建了沟叶结缕草对高温(40℃)处理响应的正向差减文库.通过reverse northern杂交方法筛选文库,挑选出高温胁迫处理下35个差异表达基因,对这些基因进行测序,并将测序所得结果与基因组数据库(NCBI)进行比对,从而获得了26个基因功能已知的差异表达基因.这些已知基因分别参与信号转导、植物代谢、植物抗逆性反应、基因表达调控和蛋白质合成等过程.
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| 关 键 词： | 沟叶结缕草 高温胁迫 抑制消减杂交 基因表达 |
| 收稿时间： | 2008-12-22 |
Analysis of Different Expression of Genes in Zoysia Matrella Exposed to High Temperature Treatment |
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| HUANG Jin-wen,WU Wen-xiang,CHEN Dong-mei,ZHENG Hong-yan,LIN Wen-xiong.Analysis of Different Expression of Genes in Zoysia Matrella Exposed to High Temperature Treatment[J].Acta Agrestia Sinica,2009,17(4):435-439. |
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| Authors: | HUANG Jin-wen WU Wen-xiang CHEN Dong-mei ZHENG Hong-yan LIN Wen-xiong |
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| Institution: | Institute of Agroecology, Fujian Agriculture and Forestry University, Fuzhou 350002, China |
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| Abstract: | Some studies on the genes of disease-resistance,insect-resistance and salt-tolerant in turfgrass have been carried out,however few information is available in the heat-tolerant genes.In order to study molecular mechanisms of heat resistance of Zoysia matrella,an investigation,detecting the related gene expression profiles exposed to high temperature stress,was conducted using suppression subtractive hybridization(SSH).After the reverse northern hybridization screening,a total of 35 ESTs from SSH-cDNA librar... |
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| Keywords: | Zoysia matrella High temperature stress Suppression subtractive hybridization(SSH) Gene expression |
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