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| 平邑甜茶与扎矮山定子杂交后代的倍性鉴定及核型分析 | |
| 引用本文： | 周攀,张景娥,王颖,周晏起,王毅,周洪富,董文轩.平邑甜茶与扎矮山定子杂交后代的倍性鉴定及核型分析[J].中国农学通报,2009,25(22):186-193. |
| 作者姓名： | 周攀 张景娥 王颖 周晏起 王毅 周洪富 董文轩 |
| 作者单位： | 1. 辽宁省果树科学研究所,辽宁熊岳,115009 2. 吉林农业大学园艺学院,长春,130118 3. 沈阳农业大学园艺学院,沈阳,110161 |
| 基金项目： | 国家自然科学基金项目 |
| 摘 要： | 为了确定平邑甜茶Malus hupehensis（Pamp.）Rehd]与扎矮山定子Malus Baccata（L.）Borkh]杂交后代的倍性特征和核型特点,以风油精为预处理液采用常规压片法对杂交后代进行染色体数目观察和核型分析,以亲本和普通山定子为对照。结果表明：供试材料中6株皱叶型株系均为四倍体,2n=4x=68。染色体均为小型,染色体长度比差异不大,核型组成由中部着丝点染色体（m）和亚中部着丝点染色体（sm）组成。株系10#、14#、15#、19#、28#、33#核型公式分别为,2n=4x=44m＋24sm,2n=4x=60m＋8sm,2n=4x=20m＋48sm,2n=4x=52m＋16sm,2n=4x=56m＋12sm,2n=4x=48m＋20sm,核型分别为1B、2B、2A、2B、1A和1B类型;1株光叶株系为非整倍体,2n=43,核型公式为2n=22m＋18sm＋3st,核型为2B类型。表现为株系之间的核型不完全相同。试验为丰富无融合生殖型砧木的育种理论,获得新的苹果无融合生殖型砧木提供有益的借鉴。 |
| 关 键 词： | 平邑甜茶 扎矮山定子 倍性鉴定 核型分析 染色体 |
| 收稿时间： | 2009/7/23 0:00:00 |
| 修稿时间： | 2009/8/24 0:00:00 |
Ploidy Identification and Karyotype Analysis of the Hybrids Crossed by Pingyi Tiancha [Malus hupehensis (Pamp.) Rehd] and Zha'ai Shangdingzi [Malus baccata (L.) Borkh.] |
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| Zhou Pan,Zhang Jing'e,Wang Ying,Zhou Yanqi,Wang Yi,Zhou Hongfu,Dong Wenxuan.Ploidy Identification and Karyotype Analysis of the Hybrids Crossed by Pingyi Tiancha [Malus hupehensis (Pamp.) Rehd] and Zha'ai Shangdingzi [Malus baccata (L.) Borkh.][J].Chinese Agricultural Science Bulletin,2009,25(22):186-193. | |
| Authors: | Zhou Pan Zhang Jing'e Wang Ying Zhou Yanqi Wang Yi Zhou Hongfu Dong Wenxuan |
| Institution: | Zhou Pan, Zhang Jing＇e , Wang Ying, Zhou Yanqi, Wang Yi, Zhou Hongfu, Dong Wenxuan (1Liaoning Research Institute of Pomology, Xiongyue Liaoning 115009; 2College of Horticulture, Jilin Agricultural University, Changchun 130118; 3College of Horticulture, Shenyang Agricultural University, Shenyang 110161) |
| Abstract: | Using the hybrids crossed by Malus hupehensis （Pamp.） Rehd and Malus baccata （L.） Borkh, the control plants of the two parents and normal M. baccata as the experimental materials, the chromosome number was observed and the karyotype was analysed by the methods of Squash with FengYouJing as pretreatment solution. The results indicated that the 6 leaf-wrinkled plants （10^#. 14^#, 15^#, 19^#, 28^#, 33^#） were all tetraploidy and composed of metacentric chromosomes and submetacentric chromosomes, 2n=4x=68, which were all small chromosomes and whose morphological variations and relative length ratios were not obvious. The karyotype formulas of them were 2n=4x=44m＋24sm, 2n=4x=60m＋8sm, 2n=4x=20m＋48sm, 2n=4x=52m＋ 16sm, 2n= 4x=56m＋ 12sm, 2n=4x=48m＋20sm. The karyotypes of the 6 leaf-wrinkled plants were 1B, 2B, 2A, 2B, 1A and 1B respectively, which indicated that the karyotypes were not identical completely among the materials. This research enriched the breeding methods and offered some new apomictic stocks in Malus for the utilization in fruit production. |
| Keywords: | Malus hupehensis（Pamp ） Rehd Malus Baccata（L ） Borkh ploidy identification karyotype analysis chromosome |
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