Characterization of single‐nucleotide polymorphism markers in the Mediterranean mussel,Mytilus galloprovincialis |
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| Authors: | Manuel Vera Belén G Pardo Ania Pino‐Querido José Antonio Álvarez‐Dios José Fuentes Paulino Martínez |
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| Institution: | 1. Departamento de Genética, Facultad de Veterinaria, Universidad de Santiago de Compostela, Campus de Lugo, Lugo, Spain;2. Departamento de Matemática Aplicada, Facultad de Matemáticas, Universidad de Santiago de Compostela, Santiago de Compostela, Spain;3. Centro de Investigacións Mari?as, Xunta de Galicia, Vilanova de Arousa, Pontevedra, Spain |
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| Abstract: | The Mediterranean mussel, Mytilus galloprovincialis, is one of the most important aquaculture species in Europe. Appropriate molecular markers are required to evaluate genetic resources and to trace genealogies in breeding programmes for improving mussel culture. Microsatellites have been commonly applied to this purpose in other species. However, Mediterranean mussel microsatellites have demonstrated high frequencies of null alleles that hamper accurate estimates of population parameters and confident parentage inferences. As alternative markers, we have characterized in silico 25 potential single‐nucleotide polymorphism (SNP) markers in the Mediterranean mussel from expressed sequence tag (EST) public databases. The genotyping of SNPs was performed using a single‐base extension approach. Their polymorphism was evaluated in 47 individuals from an Atlantic population. Out of the 25 potential SNPs tested, 12 were technically feasible (producing a single amplicon) and polymorphic. All were biallelic and had an unbiased heterozygosity ranging from 0.160 to 0.504. One SNP was from a mitochondrial gene. The combined potential of nuclear SNPs for parentage assignment gave an exclusion probability of a false couple of parents of 0.9471. These markers will be useful for evaluating resources and tracing genealogies in genetic breeding programmes implemented to solve the main problems of mussel culture. |
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| Keywords: | single‐nucleotide polymorphism SNPs SNaPshot mussel Mytilus galloprovincialis |
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