Isolation of IGF2 and association of IGF2 polymorphism with growth trait in genetically improved farmed tilapias,Oreochromis niloticus L. |
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| Authors: | Yu Juhua Chen Xuefeng Li Jianlin Tang Yongkai Li Hongxia Xu Pao Dong Zaijie |
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| Institution: | Key Laboratory for Genetic Breeding of Aquatic Animals, Aquaculture Biology Certified by the Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, China |
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| Abstract: | Molecular marker‐assisted selection increases the selection precision and reduces the number of generations required to achieve a desired improvement. Here, we report on the relationship between polymorphism in the insulin‐like growth factor II (IGF2) gene and growth trait in a genetically improved farmed tilapia strain (GIFT strain). We first isolated the entire IGF2 (5517 bp), including the complete reading frame and three interrupting introns. After sequence comparison of IGF2 from 10 individuals, we identified nine polymorphic sites in intron 1 and intron 3, and four silent mutation sites in exon 2 and exon 3. Four of the 13 polymorphic sites were examined and genotyped using various technologies, including PCR‐RFLP, tetra‐primer PCR and PCR‐PAGE among 192 experimental individuals. Regression analyses demonstrated that two sites, G161A in exon 3 and the microsatellite locus in intron 3, were significantly associated with male GIFT growth. These two putative markers will be further validated in our future breeding project. |
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| Keywords: | genetically improved farmed tilapias strain (GIFT strain) IGF2 polymorphism growth trait |
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