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An intensive hatchery rearing protocol for larvae of the bullseye puffer,Sphoeroides annulatus (Jenyns)
Authors:Maria Isabel Abdo‐de la Parra  Armando García‐Ortega  Irma Martínez‐Rodríguez  Blanca González‐Rodríguez  Gabriela Velasco‐Blanco  Crisantema Hernández  Neil Duncan
Institution:1. CIAD, A.C. Unidad Mazatlán, Sinaloa, México;2. IRTA Sant Carles de la Rapita, Tarragona, Spain
Abstract:In this study bullseye puffer, Sphoeroides annulatus larvae were reared from hatching through to 1 or 2 months after weaning on an experimental scale in three replicate 600 L tanks and on three occasions during the spawning season (nine tanks in total). The rearing protocol used was green water (Nannochloropsis oculata and Isochrysis sp.) 100 000 cells mL?1 from 0 to 11 days after hatch (DAH), 5–10 rotifers, Brachionus rotundiformis mL?1 from 2 to 26 DAH, 0.1–1 Artemia mL?1 from 21 to 34 DAH and weaning from 29 to 34 DAH. Survival to a month after weaning was 1%, a total of 3153 juveniles were produced with an average wet weight of 0.29 ± 0.07 g and a length of 27.5 ± 0.82 mm.
Keywords:bullseye puffer fish  Sphoeroides annulatus  larvae  culture  growth  survival
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