Attachment of E. coli-bearing K88 antigen to equine brush-border membranes |
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| Authors: | S Tzipori M Withers J Hayes R Robins-Browne K L Ward |
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| Institution: | “Attwood” Institute for Veterinary Research, Department of Agriculture, Mickleham Road, Westmeadows 3047, Vic. Australia;Department of Microbiology, University of Melbourne, Parkville 3052, Vic., Australia |
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| Abstract: | Equine small intestinal brush-border membranes, from 40 adult horses were tested in vitro for the presence of receptors for the Escherichia coli adhesive antigens K88ab, K88ac and K99. Only K88-positive strains of E. coli adhered strongly to horse brush-border membranes. In contrast, a K88-negative mutant strain J2, 2 K99-positive strains and 3 E. coli strains isolated from foals failed to adhere to horse brush-border membranes. Purified K88ac pili when reacted with equine brush-border membranes inhibited to a great extent the adhesion of K88-positive E. coli. Similarly, K88-positive E. coli previously reacted with K88 antibody, did not attach to equine brush-border membranes. Oral inoculation of 4 newborn foals with strains of K88-positive enterotoxigenic E. coli, producing either heat-stable or heat-labile enterotoxin, caused diarrhoea in 1 animal. |
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