| 猪传染性胃肠炎病毒YK株N基因的原核表达与N蛋白的生物学活性检测 |
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| 引用本文： | 张雷,卫广森.猪传染性胃肠炎病毒YK株N基因的原核表达与N蛋白的生物学活性检测[J].中国兽药杂志,2005,39(5):10-12,19. |
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| 作者姓名： | 张雷 卫广森 |
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| 作者单位： | 1. 沈阳农业大学畜牧兽医学院,辽宁,沈阳,110161
2. 辽宁省动物防疫站,辽宁,沈阳,110161 |
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| 摘 要： | 将猪传染性胃肠炎病毒的N基因克隆到表达载体pET28a后,转化到BL21(DE3)大肠杆菌表达系统中.通过筛选表达温度和IPTG的浓度,使N基因在原核表达系统中表达并使目的表达产物产量达到最高值.将诱导表达的产物进行SDS-PAGE蛋白电泳和Western检验,证明该表达产物是猪传染性胃肠炎病毒的N蛋白.试验结果说明,猪传染性胃肠炎病毒N基因能够在原核表达系统中大量表达,而且表达的蛋白质具有生物学活性.
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| 关 键 词： | N基因 原核表达 |
| 文章编号： | 1002-1280(2005)05-0010-04 |
Expressing by N gene of TGEV through prokaryotic cells and assaying the biology activity of N proteins |
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| ZHANG Lei,WEI Guang-sen.Expressing by N gene of TGEV through prokaryotic cells and assaying the biology activity of N proteins[J].Chinese Journal of Veterinary Drug,2005,39(5):10-12,19. |
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| Authors: | ZHANG Lei WEI Guang-sen |
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| Abstract: | After cloning into the expression vector pET28a, the N gene of transmissible gastroenteritis virus (TGEV) was transformed into BL-21(DE3) E. coli prokaryotic expression system. By selecting expression temperature and IPTG concentration, the N gene was expressed at high level in prokaryotic cells. The expression product was detected by SDS-PAGE protein electrophoresis and Western protein hybridization and had been showed that it was the N protein of TGEV. From the results of the experiments, we concluded that the N gene of TGEV can be highly expressed in prokaryotic expression system and the expression product had biology activity. cloning |
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| Keywords: | SDS-PAGE Western-blot |
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