The New Zealand apple genome mapping project |
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| Authors: | Susan E Gardiner Ji Mei Zhu Heather C M Whitehead Charlotte Madie |
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| Institution: | (1) The Horticulture and Food Research Institute of New Zealand Ltd (HortResearch), Private Bag 11030 Palmerston North, New Zealand |
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| Abstract: | Summary This project was initiated three years ago to support the New Zealand apple breeding programme at HortResearch and is funded
by both the New Zealand Foundation for Research, Science and Technology and the New Zealand Apple and Pear Marketing Board.
Our initial goal is to construct a linkage map comprising RFLP, RAPD and isoenzyme markers as well as characters of importance
to our breeders and this will enable the breeders to use marker assisted selection to identify the most promising seedlings
within progeny populations at an early age. Characters of interest in the shorter term include resistances to scab, powdery
mildew, woolly apple aphid and silver leaf, and in the longer term more complex traits such as early and late fruit maturity,
fruit quality characters, low temperature tolerance and rootstock influences such as dwarfing and precocity are under consideration.
We are currently developing long-term plans for isolation of apple genes in association with other molecular biologists in
Hort Research. We have used a cDNA library from apple flesh as a prolific source of RFLP probes and detect their hybridization
to Southern blots using a chemiluminescent method. We have screened our mapping line of seedlings for more than 160 markers
so far (1/3 RFLP, 2/3 RAPD). Detection of in situ hybridization of key RFLP probes to metaphase chromosome preparations for
the purpose of associating marker linkage groups with physical chromosomes is by a digoxygenin or biotin linked immunoassay.
The project has collaborative links to apple genome mapping projects in America and Europe. |
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| Keywords: | Apple chromosomes gene mapping Malus RAPD RFLP |
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