| 基于COⅠ基因我国鹦嘴鱼属鱼类分子系统分类关系 |
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| 引用本文： | 何浩斌,杨杰銮,陈玉佩,陈震翰,张凯,梁日深,林蠡.基于COⅠ基因我国鹦嘴鱼属鱼类分子系统分类关系[J].仲恺农业工程学院学报,2021,34(4):21-29. |
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| 作者姓名： | 何浩斌 杨杰銮 陈玉佩 陈震翰 张凯 梁日深 林蠡 |
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| 摘 要： | 为探讨鹦嘴鱼属（Scarus）鱼类分子系统分类关系，本研究对采集的16种鹦嘴鱼类COⅠ基因部分序列进行PCR扩增和测序，结合GenBank下载的2种绿鹦嘴鱼属COⅠ基因序列共同分析，利用MEGA7.0软件计算遗传距离，采用最大似然法和邻接法构建分子系统进化树．结果显示：18种鹦嘴鱼大致形成3个分支，其中青鲸鹦嘴鱼属（Cetoscarus）单独形成一支，位于进化树基部；鹦嘴鱼属进化地位较高，位于进化树顶部；绿鹦嘴鱼属（Chlorurus）进化地位介于两者之间．驼背绿鹦嘴鱼（Chlorurus gibbus）及污色绿鹦嘴鱼（Chlorurus sordidus）没有与鹦嘴鱼属聚为一支，而是与绿鹦嘴鱼属聚在一起，支持两者归为绿鹦嘴鱼属的分类观点．许氏鹦嘴鱼（Scarus schlegeli）与棕吻鹦嘴鱼（Scarus psittacus）亲缘关系十分接近，但两者COⅠ遗传距离差异为0.085，大于0.020的种间遗传距离差异临界值，两者应该是不同的物种. 截尾鹦嘴鱼（Scarus rivulatus）与黑斑鹦嘴鱼（Scarus globiceps） COⅠ遗传距离为0.017，小于0.020的临界值.但两者形态存在较大差异，排除同种异名可能性，有可能两者存在自然杂交或基因渗透．
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| 收稿时间： | 2022-01-10 |
Molecular taxonomic relationship of Scarus in China based on COⅠ gene |
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| Abstract: | To investigate the molecular phylogenetic relationships of Scarus, partial sequences of COⅠ genes from 16 parrotfish were obtain by PCR amplification and sequencing. Combining with two COⅠ sequences of Chlorurus species from NCBI, genetic distances among different parrotfish were computed using MEGA7.0 software, and the phylogenetic trees were constructed basing on Neighbor joining and Maximum likelihood method. Results showed that: 18 parrotfish mainly formed three clades, genus Cetoscarus formed a separate clade, which was located at the base of the phylogenetic tree; Genus Scarus had a high evolutionary status and was located at the top of the tree, while genus Chlorurus was between the two Cetoscarus and Scarus. Chlorurus gibbus and Chlorurus sordidus were not grouped within the genus Scarus, but were clustered inside the genus Chlorurus, which supported the taxonomic view that they were classified into Chlorurus. The phylogenetic relationship between Scarus schlegeli and Scarus psittacus was close, but the COⅠ genetic distance between them was 0.085, higher than the species identification critical value (0.020), indicating that they were two independent species. The COⅠ genetic distance between Scarus rivulatus and Scarus globiceps was 0.017, which was less than the critical value (0.020). But there were great differences in morphology, excluding the possibility of homonymy, it might be natural hybridization or gene penetration between them. |
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