| 芥菜Fosmid 文库构建及B 基因组细胞学标记的筛选利用 |
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| 引用本文： | 彭元凤,孟德璇,黄玉碧,王桂香.芥菜Fosmid 文库构建及B 基因组细胞学标记的筛选利用[J].园艺学报,2012,39(7):1313-1320. |
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| 作者姓名： | 彭元凤 孟德璇 黄玉碧 王桂香 |
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| 作者单位： | 四川农业大学玉米研究所，四川温江 611130； 北京市农林科学院蔬菜研究中心，北京 100097；中国农业大学国家玉米改良中心，北京 100193 |
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| 基金项目： | 基金项目:国家自然科学基金项目，国家‘863’计划项目，北京市科技专项，北京市农林科学院科技创新能力建设专项 |
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| 摘 要： | 构建了由60 000 个克隆组成的芥菜无偏倚Fosmid 文库，该文库外源片段插入率为100%，外源DNA 平均插入长度为32 kb，文库覆盖率约为芥菜基因组的1.8 倍。利用不同来源的分子标记筛选文库，得到的阳性单克隆经荧光原位杂交（FISH）鉴定后，获得两类B 基因组细胞学标记，一类在所有染色体上都有信号，另一类仅在一对染色体上有信号。
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| 关 键 词： | 芥菜 Fosmid 文库 细胞学标记 分子标记 FISH |
Construction of Genomic Fosmid Library of Brassicajuncea and Screening of Cytological Markers for B-genome Chromosomes |
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| PENG Yuan-feng,MENG De-xuan,HUANG Yu-bi,WANG Gui-xiang,and LIU Fan.Construction of Genomic Fosmid Library of Brassicajuncea and Screening of Cytological Markers for B-genome Chromosomes[J].Acta Horticulturae Sinica,2012,39(7):1313-1320. |
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| Authors: | PENG Yuan-feng MENG De-xuan HUANG Yu-bi WANG Gui-xiang and LIU Fan |
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| Institution: | Maize Research Institute of Sichuan Agricultural University，Wenjiang，Sichuan 611130，China；Beijing VegetableResearch Center，Beijing 100097，China；China National Maize Improvement Center of China Agricultural University，Beijing 100193，Chinan |
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| Abstract: | In this study,an unbiased Fosmid library of Brassica juncea was established,which consisted of 60 000 clones with 100% inserting frequency.In the constructed library,the size of average inserts was approximately 32 kb,corresponding to 1.8 genome equivalents.Subsequently,two types of B-genome cytological markers were identified by means of library screening and chromosome fluorescence in situ hybridization(FISH).One type was that the signal located on all chromosomes,and the other one was that the signal was detected only on one pair of chromosomes. |
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| Keywords: | Brassicajuncea fosmid library cytological marker molecular marker FISH |
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