| 番茄抗叶霉病基因Cf12 的分子标记筛选及种质资源鉴定 |
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| 引用本文： | 赵婷婷,宋宁宁,姜景彬,张贺,康立功,李景富,许向阳.番茄抗叶霉病基因Cf12 的分子标记筛选及种质资源鉴定[J].园艺学报,2012,39(5):985-991. |
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| 作者姓名： | 赵婷婷 宋宁宁 姜景彬 张贺 康立功 李景富 许向阳 |
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| 作者单位： | （东北农业大学园艺学院，哈尔滨 150030） |
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| 基金项目： | 现代农业产业技术体系专项，东北农业大学创新团队基金项目;东北农业大学博士启动基金项目，黑龙江省高等学校创新团队基金项目，哈尔滨市科技创新人才研究专项 |
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| 摘 要： | 以番茄抗叶霉病的品种05HN36为母本，以感病品种051355为父本配置杂交组合，以亲本及其F2分离群体为研究材料，采用AFLP技术筛选与抗叶霉病基因Cf12连锁的分子标记。通过对545对引物进行筛选，获得了6个连锁的AFLP标记：E86M51-C、E46M50、E95M59-D、E35M81、E78M36-C和E47M50-C，连锁遗传距离分别为5.1、8.9、9.1、9.2、10.2和10.8 cM。将E86M51-C转化为SCAR标记并应用于种质资源筛选，获得了16份含有该标记的番茄材料，为抗叶霉病育种提供材料基础。
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| 关 键 词： | 番茄 叶霉病 Cf12 基因 AFLP 分子标记 SCAR |
Screening of Molecular Markers Linked to Cladosporium fulvum Resistant Gene Cf12 in Tomato and Identification of Germplasm Resource |
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| ZHAO Ting-ting,SONG Ning-ning,JIANG Jing-bin,ZHANG He,KANG Li-gong,LI Jing-fu,and XU Xiang-yang.Screening of Molecular Markers Linked to Cladosporium fulvum Resistant Gene Cf12 in Tomato and Identification of Germplasm Resource[J].Acta Horticulturae Sinica,2012,39(5):985-991. |
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| Authors: | ZHAO Ting-ting SONG Ning-ning JIANG Jing-bin ZHANG He KANG Li-gong LI Jing-fu and XU Xiang-yang |
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| Institution: | （College of horticulture，Northeast Agricultural University，Harbin 150030，China） |
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| Abstract: | The study identified the molecular marker linked to Cladosporium fulvum resistant gene Cf12 in tomato. The linked marker was screened with a F2 population between a resistant and a susceptible parent(05HN36 × 051355)by AFLP technology. Six AFLP markers had been identified to linked with Cf12 gene:E86M51-C,E46M50,E95M59-D,E35M81,E78M36-C and E47M50-C. The genetic distances were 5.1,8.9,9.1,9.2,10.2 and 10.8 cM respectively. The AFLP marker E86M51-C was converted into SCAR marker. We obtained 16 cultivars which contained this marker. These cultivars could be used in C. fulvum resistant breeding practice. |
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| Keywords: | tomato Cladosporium fulvum resistance gene Cf12 AFLP SCAR |
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