| 过量表达tMEK2基因的大岩桐植株对温度胁迫的反应 |
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| 引用本文： | 孙姝兰,温秋香,代建丽,邢惕,王小菁.过量表达tMEK2基因的大岩桐植株对温度胁迫的反应[J].园艺学报,2012,39(2):375-379. |
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| 作者姓名： | 孙姝兰 温秋香 代建丽 邢惕 王小菁 |
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| 作者单位： | 1华南师范大学生命科学学院，广东省植物发育生物工程重点实验室，广州 510631；2卡尔顿大学生物系，渥太华 K1S5B6，加拿大 |
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| 基金项目： | 国家自然科学基金青年基金项目，广东省自然科学基金重点项目 |
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| 摘 要： | 构建番茄中MAPKK基因tMEK2的过量表达载体并转化到大岩桐中，通过PCR和Southern blot鉴定出转基因株系并对其进行抗性分析。结果表明，转基因植株与对照相比叶片变小，叶片的叶绿素含量显著增加，在4 ℃低温和40 ℃高温胁迫之后的恢复能力明显比对照增强。
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| 关 键 词： | 大岩桐 tMEK2 过量表达 温度胁迫 恢复 |
| 收稿时间： | 2011-11-01 |
Responses of Sinningia speciosa Overexpressing tMEK2 to Temperature Stresses |
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| SUN Shu-lan,WEN Qiu-xiang,DAI Jian-li,XING Tim,and WANG Xiao-jing.Responses of Sinningia speciosa Overexpressing tMEK2 to Temperature Stresses[J].Acta Horticulturae Sinica,2012,39(2):375-379. |
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| Authors: | SUN Shu-lan WEN Qiu-xiang DAI Jian-li XING Tim and WANG Xiao-jing |
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| Institution: | 1. College of Basic Medicine,Yanbian University, Yanji 133002, China;
2. College of Pharmacy, Yanbian University, Yanji 133002, China |
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| Abstract: | We constructed a MAPKK gene from tomato,tMEK2 overexpressing construct to transform into Sinningia speciosa.Transgenic plants were identified by PCR and Southern blotting analyses,and their resistance to cold and high temperatures was analyzed.The results showed that the tMEK2 overexpressing plants had smaller leaves,increased contents of chlorophyll in leaves,and better recoveries of plant growth after treatments with cold and high temperatures,compared to control of wild type plants. |
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| Keywords: | Sinningia speciosa tMEK2 overexpression temperature stress recovery of growth |
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