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抗氧化酶活性作为植物抗盐性 评价指标的有效性
引用本文:刘强,王庆成,王占武.抗氧化酶活性作为植物抗盐性 评价指标的有效性[J].广东农业科学,2014,41(24):49-53.
作者姓名:刘强  王庆成  王占武
作者单位:吉林师范大学生命科学学院,吉林四平,136000
基金项目:吉林省科技发展计划项目,四平市科技发展计划项目,吉林师范大学博士科研启动基金
摘    要:以5个抗盐性不同的树种河南白榆、新疆枸杞、南蛇藤、小叶丁香、樟子松的1年生实生苗为试验材料,通过测定盐胁迫下5个树种叶片丙二醛含量和超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)、抗坏血酸氧化酶(APX)等抗氧化酶活性的变化,研究抗氧化酶活性作为植物抗盐性评价指标的有效性.结果表明:盐胁迫下5个树种叶片丙二醛含量显著增加,丁香和樟子松增加幅度明显大于枸杞和白榆.SOD、CAT可以作为不同树种抗盐性评价标准,其中SOD、CAT更为有效.各胁迫时期抗盐性较强的枸杞和白榆SOD活性随胁迫强度的增加逐渐升高,而抗盐性较弱的丁香和樟子松SOD活性却不断下降;长期胁迫下抗盐性较强的枸杞和白榆CAT活性随胁迫强度的增加逐渐升高,而抗性相对较弱的丁香和樟子松CAT活性轻度胁迫下升高,较重胁迫下却降低.POD和APX不是评价不同树种抗盐性的可靠指标.POD活性增加是一种胁迫症状,而不是一种抗性表现.随胁迫强度的增加,抗盐碱性较弱的丁香和樟子松POD活性增加,而抗盐碱性较强的枸杞和白榆POD活性却降低.

关 键 词:抗氧化酶  抗盐性  评价指标  有效性

Effectiveness of antioxidant enzymes as evaluation index for salt tolerance of plants
LIU Qiang,WANG Qing-cheng,WANG Zhan-wu.Effectiveness of antioxidant enzymes as evaluation index for salt tolerance of plants[J].Guangdong Agricultural Sciences,2014,41(24):49-53.
Authors:LIU Qiang  WANG Qing-cheng  WANG Zhan-wu
Abstract:The effectiveness of activitiy of antioxidant enzymes as evaluation index for salt tolerance were investigated in five trees differing in tolerance to salt stress by determining the malondialdehyde (MDA) content and the activities of antioxidant enzymes like superoxide dismutase (SOD), catalase (CAT), peroxidase (POD) and ascorbate peroxidase (APX). The results showed that MDA contents of the five trees increased significantly under salt stress. The increasing scales of MDA contents of Syringa microphylla and Pinus sylvestris var. mongolica were larger than that of Ulmus pumila and Lycium dasystemum. The SOD and CAT could serve as reliable indicators for appraising salt tolerance of woody plants, and SOD and CAT were more effective. With the increase of stress intensity, the SOD activities of U. pumila and L. dasystemum increased, while those of S. microphylla and P. sylvestris var. mongolica decreased obviously. POD and APX were not reliable selction criteria. Increase in POD activity did not seem to be a tolerance component, but a stress symptom.
Keywords:antioxidant enzyme  salt tolerance  evaluation index  effectiveness
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