| 丽蚌草组织培养及植株再生 |
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| 引用本文： | 宛淑艳,刘金祥,侯玉婷.丽蚌草组织培养及植株再生[J].广东农业科学,2014,41(24):45-48. |
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| 作者姓名： | 宛淑艳 刘金祥 侯玉婷 |
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| 作者单位： | 岭南师范学院生命科学与技术学院,广东湛江,524048 |
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| 基金项目： | 国家级星火计划项目，广东高校边缘热带特色植物工程开发中心项目，湛江市财政资金科技专项，湛江市“热带特色资源植物技术开发”重点实验室项目 |
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| 摘 要： | 以丽蚌草(Arrhenatherum elatius var.tuberosum ‘Variegatum’)茎叶连接的组织为外植体,探讨不同培养基对丽蚌草愈伤组织的诱导、生芽诱导及生根的影响.结果表明:当培养基为B5+2,4-D 4 mg/L+CH 0.3 g/L时,丽蚌草叶片诱导愈伤组织效果最好;当培养基为MS+6-BA 5 mg/L+NAA 2 mg/L时,丽蚌草愈伤组织生芽效果较好;当培养基为1/2MS+6-BA 0.1 mg/L+NAA 1.0 mg/L+0.1％活性炭时,生根效果最好.
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| 关 键 词： | 丽蚌草 愈伤组织 诱导生芽 诱导生根 |
Tissue culture and plant regeneration
of Arrhenatherum elatius |
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| WAN Shu-yan,LIU Jin-xiang,HOU Yu-ting.Tissue culture and plant regeneration
of Arrhenatherum elatius[J].Guangdong Agricultural Sciences,2014,41(24):45-48. |
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| Authors: | WAN Shu-yan LIU Jin-xiang HOU Yu-ting |
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| Abstract: | The influences of different culture medium on the induced callus, induced buds and roots of Arrhenatherum
elatius with the leaves as explants were explored. The results show that the optimum induced callus medium was B5+2,4-D
4 mg/L+CH 0.3 g/L; the optimum induced buds medium was MS+6-BA 5 mg/L+NAA 2 mg/L and the optimum induced
roots medium was 1/2MS+6-BA 0.1 mg/L+NAA 1.0 mg/L+0.1% acticarbon. |
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| Keywords: | Arrhenatherum elatius callus induced buds induced roots |
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