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| 鸡毒支原体C株的分离鉴定及与其它北京株结构蛋白的比较分析 | |
| 引用本文： | 苗得园,陈德威,张大华,陈小玲.鸡毒支原体C株的分离鉴定及与其它北京株结构蛋白的比较分析[J].中国兽医杂志,1998,24(8):5-7. |
| 作者姓名： | 苗得园 陈德威 张大华 陈小玲 |
| 作者单位： | 1. 北京市农林科学院畜牧兽医研究所,北京海淀,100081 2. 中国农业大学动物医学院,北京,100094 3. 山东省淮坊市寒亭区畜牧局,261109 |
| 摘 要： | 本文对北京某鸡场气囊炎病进行了支原体的分离和鉴定，并将此分离株与北京另两个分离株ＢＧ４４Ｔ、ＮＢ７２的结构蛋白通过ＳＤＳ－ＰＡＧＥ进行了比较分析。结果表明此分离株（命名为Ｃ株）可发酵葡萄糖，不水解尿素和利用精氨酸，是一具有毒力较强的鸡毒支原体株。ＳＤＳ－ＰＡＧＥ结果表明，此分离株与ＮＢ７２株相当一致，与ＢＧ４４Ｇ株有相对较大的区别。我们认为，Ｃ株和ＮＢ７２株具有同源性，鸡胚液制造的活疫苗中支原体的污染可能源于鸡胚培养时采用了ＭＧ感染病鸡所产蛋，另外，还可看出北京地区的支原体感染存在着一定的多样性。 |
| 关 键 词： | 鸡毒支原体 分离鉴定 SDS-PAGE 结构蛋白 |
Isolation and Identification of A Field Mycoplasma Gallisepticum Strain and Comparative Analysis of its Structural Proteins with Other Beijing Strains |
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| Miao deyuan,Chen Dewei,Zhang Dahua,Chen Xiaoling.Isolation and Identification of A Field Mycoplasma Gallisepticum Strain and Comparative Analysis of its Structural Proteins with Other Beijing Strains[J].Chinese Journal of Veterinary Medicine,1998,24(8):5-7. | |
| Authors: | Miao deyuan Chen Dewei Zhang Dahua Chen Xiaoling |
| Abstract: | A strain of Mycoplasma gallisepticum(named C)was isolated and identified,the structural proteins of this strain was compared and analyzed with two other Beijing MG strains BG 44 T and NB 72 by SDS PAGE. The results indicated that this islate could ferment glocuse,failed to hydrolyse urea or use arginine,and also had a strong virulence. The results of SDS PAGE showed that,all these three strains had similar SDS PAGE patterns,and strains C and NB 72 presented exactly the same patterns,while they both had a relatively obvious distinction with BG 44 T.So we could conclude that strain C and NB 72 were homologous,this suggests that the sources of contamination with MG in live virus vaccine made from chicken embryos might be contaminated egs from infected hens.Moreover,the MG infection in Beijing had a certain degree of varieties. |
| Keywords: | Mycoplasma gallisepticum Isolation and identification SDS PAGE Structural proteins |
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