Changes in digestive enzyme activity during initial ontogeny of bay snook Petenia splendida |
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| Authors: | Uscanga-Martínez A Perales-García N Alvarez-González C A Moyano F J Tovar-Ramírez D Gisbert G E Márquez-Couturier G Contreras-Sánchez W M Arias-Rodríguez L Indy J R |
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| Institution: | (1) Laboratorio de Acuicultura Tropical, DACBIOL, Universidad Ju?rez Aut?noma de Tabasco, Carretera Villahermosa C?rdenas km 0.5, 86139 Villahermosa, Tabasco, Mexico;(2) Laboratorio de Bioqu?mica, Centro de Investigaciones Biol?gicas del Noroeste (CIBNOR), Mar Bermejo 195, Col. Playa Palo de Santa Rita, 23090, La Paz, B.C.S., Mexico;(3) IRTA, Sant Carles de la R?pita, Crta. Poble Nou km 5.5, 43540 Sant Carles de la Rapita (Tarragona), Spain;(4) Departamento de Biolog?a Aplicada, Escuela Polit?cnica Superior, La Ca?ada de San Urbano, Universidad de Almer?a, 04120 Almer?a, Spain; |
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| Abstract: | Several samples of P. splendida larvae were obtained from eggs until day 60 after hatching (dah) to determine acid and alkaline proteases, trypsin, chymotrypsin,
leucine aminopeptidase, α-amylase, lipase, and acid and alkaline phosphatase activities using biochemical techniques. Additionally,
SDS–PAGE alkaline protease zymogram and PAGE acid protease zymogram were carried out to identify active isoforms during larviculture.
Alkaline protease and chymotrypsin were present at the moment of hatching, increased gradually reaching the maximum values
at 35 dah. Trypsin and leucine aminopeptidase activities were low from hatching, increasing gradually as larvae grew. Alkaline
protease zymogram showed four zymogens, which appears at different days, remaining present until the end of the larviculture
(95.2 kDa at 11 dah, 26.4 kDa at 9 dah, 21.4 kDa at 3 dah, and 23.3 kDa at hatching). Pepsin activity was present at day 7
after hatching and increased progressively until the end of the larviculture. Acid protease zymogram only showed one zymogen
(0.65 rf), which appear at 6 dah. Lipase was high at the time of hatching and increased until 15 dah, after which decreased
gradually. Amylase was high from the beginning and until 15 dah and then decreased rapidly to almost nothing onward. Alkaline
and acid phosphatases presented a high activity at the egg stage, fell slightly during the first feeding and increased again
from 20 to 30 dah. Results obtained in this study show that larvae can be fed artificial diets starting on day 10 after hatching. |
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