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| 盐酸倍他洛尔温敏型眼用原位凝胶的制备及其含量测定 | |
| 引用本文： | 王慧娟,徐原,杜永丽,赵致,周英.盐酸倍他洛尔温敏型眼用原位凝胶的制备及其含量测定[J].贵州大学学报(农业与生物科学版),2011,30(6):517-522. |
| 作者姓名： | 王慧娟 徐原 杜永丽 赵致 周英 |
| 作者单位： | 1. 贵州大学生命科学学院,贵州贵阳550025;贵州大学中药天然药物研发中心,贵州贵阳550025;贵州省中药材繁育与种植重点(工程)实验室,贵州贵阳550025 2. 贵州省大环化学及超分子化学重点实验室,贵州贵阳,550025 3. 贵州省大环化学及超分子化学重点实验室,贵州贵阳550025;贵州省中药材繁育与种植重点(工程)实验室,贵州贵阳550025 |
| 基金项目： | 贵州省科学技术基金资助项目[黔科合J字(2008)2145号];黔科合人才团队[(2010)4006]黔科合计Z字[(2010)4015] |
| 摘 要： | 以泊洛沙姆407(F127)和泊洛沙姆188(F68)作为原位凝胶载体材料,研究胶凝温度优化处方,并采用高效波相色谱法测定原位凝胶中盐酸倍他洛尔的含量(色谱柱为ZorbaxSB-C18柱,流动相为乙腈-pH3.0磷酸盐缓冲液(45∶55,(V/V)),检测波长为280nm,流速0.8mL/min).结果表明:原位凝胶最... |
| 关 键 词： | 盐酸倍他洛尔 温度敏感原位凝胶 泊洛沙姆 制备 含量测定 |
Preparation and Content Determination of Thermosensitive in situ Gel of Betaxolol Hydrochloride for Ophthalmic Use |
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| WANG Hui-juan , XU Yuan , DU Yong-li , ZHAO Zhi , ZHOU Ying.Preparation and Content Determination of Thermosensitive in situ Gel of Betaxolol Hydrochloride for Ophthalmic Use[J].Journal of Mountain Agriculture & Biology,2011,30(6):517-522. | |
| Authors: | WANG Hui-juan XU Yuan DU Yong-li ZHAO Zhi ZHOU Ying |
| Institution: | 1,2,3*(1.College of Life Science,Guizhou University,Guiyang Guizhou 550025,China;2.Research and Development Center of Chinese Herbal Medicine and natural Medicine of Guizhou University,Guiyang Guizhou 550025,China;3.Key Laboratory of Chinese Herbal Medicine Planting and Breeding of Guizhou Province,Guiyang Guizhou 550025,China) |
| Abstract: | Poloxamer 407（ F127 ） and poloxamer 188 （F68） were used to prepare the in situ gel, and the formulation was optimized by determining the gelation temperature. HPLC method was established to determine the content of betaxolol hydroehloride in the gel. The separation was performed on a Zorbax SB-C18 column and the mobile phase consisted of aeetonitrile and pH 3.0 phosphate buffer in the ratio of 45：55 （v/v）. The UV detector wavelength was set at 280 nm with a flow rate of 0.8 ml/min. The results suggested that the best composition of the gel formulation was 20% F127 and 5% F68. The gelation temperature before and after simulated tear fluid dilution was 28.2℃ and 33.4%, respectively. The linear range was from 11.0 μg · mL^-1 to 440.0 μg · mL^-1（ r = 0. 999 7 ） and the average recovery rate was 99.5%. The method showed high precision of within-day and between-day for determination. The thermosensitive in situ gel showed a potential for the ocular drug delivery system. The established method was simple, accurate and reliable for the determination of betaxolol hydrochloride in the gel. |
| Keywords: | Betaxolol hydrochloride Thermosensitive in situ gel Poloxamer preparatio content determination |
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