| 黄杨叶栒子组织培养与快速繁殖技术研究 |
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| 引用本文： | 毕海林,和加卫,杨正松,杨洪涛,和文佳,李燕.黄杨叶栒子组织培养与快速繁殖技术研究[J].西南农业学报,2012,25(1):343-345. |
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| 作者姓名： | 毕海林 和加卫 杨正松 杨洪涛 和文佳 李燕 |
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| 作者单位： | 云南省农业科学院高山经济植物研究所,云南丽江,674100 |
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| 基金项目： | 云南省农业科学院基金资助项目“乡土园林树种—栒子资源的开发利用研究” |
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| 摘 要： | 采用组织培养技术,以幼嫩茎段为接种外植体,对黄杨叶栒子进行快速繁殖研究。结果表明,1/2 MS+6-BA 1.0 mg/L+IBA 0.1 mg/L为黄杨叶栒子较佳的启动培养基,MS+6-BA 1.5 mg/L+IBA 0.1 mg/L为较佳的增殖培养基,在生根阶段,以1/2MS+IBA 2.0 mg/L+NAA 0.5 mg/L为较佳培养基,一定量的IBA和NAA互作时,黄杨叶栒子的生根率最高,生根时间最短。
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| 关 键 词： | 黄杨叶栒子 幼嫩茎段 组织培养 生根 |
Study on Tissue Culture and Rapid Propagation of Cotoneaster buxifolius |
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| BI Hai-lin
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HE Jia-wei
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YANG Zheng-song
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YANG Hong-tao
,
HE Wen-jia
,
LI Yan.Study on Tissue Culture and Rapid Propagation of Cotoneaster buxifolius[J].Southwest China Journal of Agricultural Sciences,2012,25(1):343-345. |
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| Authors: | BI Hai-lin HE Jia-wei YANG Zheng-song YANG Hong-tao HE Wen-jia LI Yan |
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| Institution: | (Institute of Alpine Economic Plant,Yunnan Academy of Agricultural Sciences,Yunnan Lijiang 674100,China) |
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| Abstract: | The techniques of tissue culture and rapid propagation on Cotoneaster buxifolius were studied by using young stem with single bud as explants.The results showed that the initiating medium was 1/2 MS+6-BA 1.0 mg/L+IBA 0.1 mg/L,the subculture medium was MS+6-BA 1.5 mg/L+IBA 0.1 mg/L,the rootage medium was 1/2MS+IBA 2.0 mg/L+NAA 0.5 mg/L and rooting rate was the highest and rooting time was the shortest for Cotoneaster buxifolius when some measure of IBA and NAA interacted in the medium. |
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| Keywords: | Cotoneaster buxifolius Young stem Tissue culture Radication |
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