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| 不同基因型玉米幼胚愈伤诱导及植株再生研究 | |
| 引用本文： | 吴红,谢树章,林清,雷开荣,邱正高,张亚勤,王楠,周幼昆.不同基因型玉米幼胚愈伤诱导及植株再生研究[J].西南农业学报,2012,25(2):385-389. |
| 作者姓名： | 吴红 谢树章 林清 雷开荣 邱正高 张亚勤 王楠 周幼昆 |
| 作者单位： | 1. 重庆市农业科学院生物技术研究中心,逆境农业研究重庆市市级重点实验室,重庆401329 2. 重庆市农业科学院玉米所,重庆,401329 3. 北京市第二中学,北京,100009 |
| 基金项目： | 重庆市科委重点攻关项目，重庆市科委重点攻关项目，重庆市科委重点攻关项目，重庆市公益性科研院所基本科研业务费专项“抗逆转基因玉米育种材料的创制及鉴定研究” |
| 摘 要： | 本研究通过对不同基因型的玉米幼胚进行愈伤组织诱导实验,从49份玉米自交系材料中筛选出了5个胚性愈伤组织诱导率较高的自交系,其中,K6-64,K6-39,DL283-1诱导出的Ⅱ型胚性愈伤,具有很强的长期继代能力和再生能力,适宜作为转化受体材料进行遗传转化研究。对玉米幼胚在离体培养过程中影响因素进行了研究,1.5～2.0 mg/L 2,4-D能够较好的诱导玉米幼胚产生胚性愈伤组织。一定浓度的6-BA和KT都能够较好的促进玉米愈伤组织分化。 |
| 关 键 词： | 玉米自交系 幼胚 胚性愈伤组织 再生 |
Study on Callus Induction and Plantlet Regeneration from Immature Embyro among Different Maize Inbreds |
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| WU Hong , XIE Shu-zhang , LIN Qing , LEI Kai-rong , QIU Zheng-gao , ZHANG Ya-qin , WANG Nan , ZHOU You-kun.Study on Callus Induction and Plantlet Regeneration from Immature Embyro among Different Maize Inbreds[J].Southwest China Journal of Agricultural Sciences,2012,25(2):385-389. | |
| Authors: | WU Hong XIE Shu-zhang LIN Qing LEI Kai-rong QIU Zheng-gao ZHANG Ya-qin WANG Nan ZHOU You-kun |
| Institution: | 1.Biotechnology Research Center,Key Laboratory of Adversity Agriculture,Chongqing Academy of Agricultural Sciences,Chongqing 401329,China;2.Institute of Maize,Chongqing Academy of Agricultural Sciences, Chongqing 401329,China;3.Beijing No.2 Middle School,Beijing 100009,China) |
| Abstract: | In this study,6 inbred lines,which had higher inducing rate of embryogenic callus from 49 maize inbred lines by callus induction of immature embyro among different maize inbreds,were screened out.The type II callus of K6-64,K6-39,DL283-1,were suitable asreceptor material for transgenic research because of bright yellow and granular and had strong ability of long-term subculture and regeneration.In addition,the factors during in vitro culture of maize immature embryos were analyzed.The results showed that 1.5-2.0mg/L 2,4-D could induce maize immature embryos to produce embryogenic callus more effectively.A certain concentration of 6-BA and KT were able to promote maize callus differentiation,and there was no significant difference. |
| Keywords: | Maize inbred lines Immature embyro Embryogenic callus Regeneration |
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