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菟丝子寄生真菌YTJ-8的分离和鉴定
引用本文:周浩,黄宁珍,郭伦发,王新桂,龚庆芳,何金祥.菟丝子寄生真菌YTJ-8的分离和鉴定[J].西南农业学报,2012,25(1):157-160.
作者姓名:周浩  黄宁珍  郭伦发  王新桂  龚庆芳  何金祥
作者单位:广西壮族自治区/中国科学院广西植物研究所,广西桂林,541006
基金项目:中国科学院广西植物研究所基本业务费项目(桂植业09008)
摘    要:对广西靖西寄生植物菟丝子上分离的生防真菌进行研究,为菟丝子的生物防治提供依据。采集寄生于豆科羊蹄甲属植物羊蹄甲上具有炭疽病斑的日本菟丝子,分离病原并扩大培养,进行致病性鉴定。柯赫氏法则证明该病由真菌YTJ-8侵染引起;根据YTJ-8的形态学特征和ITS序列分析比较,推定其为尖孢炭疽菌(Colletotrichum acutatum)。

关 键 词:菟丝子  寄生真菌  分离  鉴定  尖孢炭疽菌

Isolation and Identification of Parasitic Fungus YTJ-8 on Dodder
ZHOU Hao , HUANG Ning-zhen , GUO Lun-fa , WANG Xin-gui , GONG Qing-fang , HE Jin-xiang.Isolation and Identification of Parasitic Fungus YTJ-8 on Dodder[J].Southwest China Journal of Agricultural Sciences,2012,25(1):157-160.
Authors:ZHOU Hao  HUANG Ning-zhen  GUO Lun-fa  WANG Xin-gui  GONG Qing-fang  HE Jin-xiang
Institution:(Guangxi Institute of Botany,Guangxi Guilin 541006,China)
Abstract:Aimed at providing scientific evidence for biological prevention and control of dodder,bio-control fungus isolated from dodder belonging to parasitic plants was studied in this paper.The Colletorichum strains were isolated from Cuscuta japonica that parasitized Bauhinia variegate,and then pathogens were isolated and cultivated.Koch’s postulation proved that the disease was caused by fungus YTJ-8 infection.According to morphology and ITS sequence comparison and analysis,the strain YTJ-8 was identified as Colletotrichum acutatum.
Keywords:Dodder  Parasitic fungi  Isolation  Identification  Colletotrichum acutatum
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