| 莲子草假隔链格孢SF-193固体培养基组分的响应曲面法优化 |
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| 引用本文： | 李庆辉,陈志谊,聂亚锋.莲子草假隔链格孢SF-193固体培养基组分的响应曲面法优化[J].西南农业学报,2012,25(1):261-265. |
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| 作者姓名： | 李庆辉 陈志谊 聂亚锋 |
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| 作者单位： | 1. 南京农业大学植物保护学院,江苏南京210095;江苏省农业科学院植物保护研究所,江苏南京210014
2. 江苏省农业科学院植物保护研究所,江苏南京,210014 |
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| 基金项目： | 江苏省高技术项目(GB2007334);国家科技支撑计划项目(2006BAD08A02) |
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| 摘 要： | 为获得空心莲子草生防菌SF-193固体培养基的最佳配方,本研究采用响应曲面法(response surface methodology,RSM)优化空心莲子草生防菌SF-193固体培养基各组分的配比,探讨各组分(大米粉,大豆粉,胡萝卜粉)对菌丝生长的影响。结果表明,大豆粉和胡萝卜粉对生防菌SF-193菌丝生长的线性效应显著,大米粉分别与大豆粉、胡萝卜粉之间的交互作用显著。固体培养基的最佳配方为大米粉61.7 g/L,大豆粉9.0 g/L,胡萝卜粉1.1 g/L。
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| 关 键 词： | 莲子草假隔链格孢SF-193 固体培养基 响应曲面法 优化 |
Components Optimization of Solid-Solid Medium for Nimbya alternantherae Fermentation |
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| LI Qing-hui
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CHEN Zhi-yi
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NIE Ya-feng.Components Optimization of Solid-Solid Medium for Nimbya alternantherae Fermentation[J].Southwest China Journal of Agricultural Sciences,2012,25(1):261-265. |
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| Authors: | LI Qing-hui CHEN Zhi-yi NIE Ya-feng |
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| Institution: | 1.Department of Plant Pathology College of Plant Protection,Nanjing Agricultural University,Jiangsu Nanjing 210095, China;2.Institute of Plant Protection,Jiangsu Academy of Agricultural Sciences,Jiangsu Nanjing 210014,China) |
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| Abstract: | Response surface methodology(RSM) was applied to optimize composition ratio of solid-medium fermented Nimbya alternantherae SF-193 and evaluate the combined effects of the three main independent parameters,rice flour,soymeal and carrot meal on mycelium growth.The results showed that the interaction between soymeal and carrot meal was significantly positive effect on the growth of mycelium.And the combined effects of rice flour with other components were also significant.The optimum concentration of rice flour,soymeal and carrot meal in solid-medium were found to be 61.7 g/L, 9.0 g/L and 1.1 g/L,respectively. |
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| Keywords: | Nimbya alternantherae SF-193 Solid-medium RSM Optimization |
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