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| 江苏省瓜蒌根结线虫种类鉴定 | |
| 引用本文： | 赵雷,张红艳,万景旺,叶小云,蒋祝成,程兆榜,周益军,魏利辉.江苏省瓜蒌根结线虫种类鉴定[J].西南农业学报,2012,25(2):517-520. |
| 作者姓名： | 赵雷 张红艳 万景旺 叶小云 蒋祝成 程兆榜 周益军 魏利辉 |
| 作者单位： | 1. 江苏农业科学院植物保护研究所,江苏南京,210014 2. 江苏省铜山县农业局,江苏徐州,221100 3. 中国矿业大学,江苏徐州,221100 4. 新洋农场,江苏射阳,224300 |
| 摘 要： | 本文对江苏瓜蒌上根结线虫样本进行广泛采集,单卵块纯化培养后,运用形态学方法和分子生物学方法对其进行了鉴定。根据雌虫会阴花纹、雌虫、雄虫和2龄幼虫的相关测量值等形态学鉴定特征,并结合特异引物的PCR扩增图谱,鉴定出该病原为南方根结线虫(Meloidogyne incognita)。结果表明,江苏省瓜蒌根结线虫群体类型为南方根结线虫,将进一步为该病害传播、流行和控制提供可行的理论依据。 |
| 关 键 词： | 瓜蒌 会阴花纹 分子鉴定 南方根结线虫 |
Identification of Root-knot Nematodes on Trichosanthes spp.in Jiangsu |
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| ZHAO Lei , ZHANG Hong-yan , WAN Jing-wang , YE Xiao-yun , JIANG Zhu-cheng , CHENG Zhao-bang , ZHOU Yi-jun , WEI Li-hui.Identification of Root-knot Nematodes on Trichosanthes spp.in Jiangsu[J].Southwest China Journal of Agricultural Sciences,2012,25(2):517-520. | |
| Authors: | ZHAO Lei ZHANG Hong-yan WAN Jing-wang YE Xiao-yun JIANG Zhu-cheng CHENG Zhao-bang ZHOU Yi-jun WEI Li-hui |
| Institution: | 1 (1.Institute of Plant Protection,Jiangsu Academy of Agricultural Sciences,Jiangsu Nanjing 210014,China;2.Agricultural Bureau of Tongshan County,Jiangsu Xuzhou 221100,China;3.China University of Mining and Technology,Jiangsu Xuzhou 221100, China;4.Xinyang Farm,Jiangsu Sheyang 224300,China) |
| Abstract: | In this paper,Meloidogyne populations on Trichosanthes spp.were collected and purified in Jiangsu.Based on diagnostic characters and morphometrics of females,males,and second-stage juveniles,the perineal patterns of female,using molecular biological techniques,the root-knot nematodes isolated from Trichosanthes were identified as Meloidogyne incognita with morphology and molecular biology.The results showed that the identification of Trichosanthes spp.which occurred on in Jiangsu was Meloidogyne incognita,which would provid theoretical basis for control of the root-knot nematodes disease. |
| Keywords: | Trichosanthes spp Perineal pattern Molecular identification Meloidogyne incognita |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |