| 西北地区主栽豌豆品种SSR指纹图谱构建 |
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| 引用本文： | 侯万伟,严清彪,张小田,刘玉皎.西北地区主栽豌豆品种SSR指纹图谱构建[J].西南农业学报,2012,25(1):240-242. |
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| 作者姓名： | 侯万伟 严清彪 张小田 刘玉皎 |
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| 作者单位： | 青海省农林科学院,青海西宁,810016 |
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| 基金项目： | 现代农业产业技术体系建设专项(CARS-09);青海高原作物种质资源创新与利用国家重点实验室培养基金;青海省高校“135高层次人才培养工程”专项 |
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| 摘 要： | 采用SSR标记技术对7个西北地区主栽豌豆品种基因组DNA进行扩增,构建了不同品种豌豆的指纹图谱。结果表明,21对不同的SSR引物中有6对扩增出多态性DNA片段,其中1对引物PSAC75可使每一品种具有其自身的特征谱带。因此,SSR技术可从DNA水平鉴别豌豆品种的分子差异,从而为分子标记辅助育种提供技术依据。
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| 关 键 词： | 豌豆 SSR 指纹图谱 |
Construction of Fingerprinting Map for Seven Pea Cultivars by SSR Marker |
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| HOU Wan-wei
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YAN Qing-biao
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ZHANG Xiao-tian
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LIU Yu-jiao.Construction of Fingerprinting Map for Seven Pea Cultivars by SSR Marker[J].Southwest China Journal of Agricultural Sciences,2012,25(1):240-242. |
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| Authors: | HOU Wan-wei YAN Qing-biao ZHANG Xiao-tian LIU Yu-jiao |
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| Institution: | (Qinghai Academy of Agriculture & Forestory,Qianghai Xining 810016,China) |
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| Abstract: | The genome DNA of the main cultivated pea cultivars in Northwest China was amplified by SSR to construct the fingerprint.The results showed that 6 pairs SSR primers of 21 pairs could amplify polymorphic DNA fragments,among which PSAC75 could make every cultivar have own characteristic spectra.Based on this,the SSR technique could identify the differences of pea cultivars in the DNA level and provide the technical support for molecular marker-assisted breeding. |
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| Keywords: | Pea SSR Fingerprinting |
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