| 广西红树林海洋微生物的分离及抗白色念珠菌的快速筛选 |
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| 引用本文： | 洪亮,杨建,解修超,陈绍兴,林丽飞.广西红树林海洋微生物的分离及抗白色念珠菌的快速筛选[J].西南农业学报,2012,25(1):140-143. |
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| 作者姓名： | 洪亮 杨建 解修超 陈绍兴 林丽飞 |
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| 作者单位： | 1. 河学院生命科学与技术学院,云南蒙自,661100
2. 陕西理工学院陕西省资源生物重点实验室,陕西汉中,723000 |
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| 基金项目： | 863海洋生物技术主题项目资助(2004AA628040);科技部社会公益项目资助(2004DIB3J072) |
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| 摘 要： | 利用一种基于96孔板的快速筛选抗白色念珠菌活性的美蓝—酶标仪检测法对从广西山口、防城、北海三个地点红树林分离的498株细菌,299株真菌进行活性检测。结果表明,157株细菌具有抗白色念珠菌活性,占总分离细菌数的31.5%,88株真菌具有抗白色念珠菌活性,占总分离真菌数的29.4%。且抗白色念珠菌活性菌株的比例与样品来源密切相关。
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| 关 键 词： | 红树林 海洋微生物 抗白色念珠菌 美蓝—酶标仪检测法 |
Isolation of Marine Microorganism from Guangxi Mangrove and Rapid Screening for Activity against Candida albicans |
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| HONG Liang
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YANG Jian
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XIE Xiu-chao
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CHEN Shao-xing
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LIN Li-fei.Isolation of Marine Microorganism from Guangxi Mangrove and Rapid Screening for Activity against Candida albicans[J].Southwest China Journal of Agricultural Sciences,2012,25(1):140-143. |
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| Authors: | HONG Liang YANG Jian XIE Xiu-chao CHEN Shao-xing LIN Li-fei |
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| Institution: | 1 (1.College of Life Sciences and Technology,Honghe University,Yunnan Mengzi 661100,China; 2.Shanxi Key Laboratory of Resource Biology,Shanxi University of Technology,Yunnan Hanzhong 723001, China) |
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| Abstract: | Methylene blue-microELISA spectrophotomer detection method was used for detecting activity against C.albicans based on 96-well plate and marine microorganisms were isolated from Guangxi mangrove in Shankou,Fangcheng,Beihai.The result showed that 157 out of 498 isolates showed activity against C.albicans,which was 31.5 % of the total bacteria isolates.Eighty-eight out of 299 strains of fungi isolates showed activity against C.albicans,which was 29.4 % of the total fungi isolates.The proportion of anti-Candida albicans active strains was closely correlated with sample collections. |
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| Keywords: | Mangrove Marine microorganism Anti-Candida albicans Methylene blue-microELISA spectrophotomer detection method |
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