| 一株小白鼠血源病原菌的分离、鉴定及生物学特性研究 |
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| 引用本文： | 陈明,李健,廖洪敏,韦柳青,石云良,张为宇,黄维义.一株小白鼠血源病原菌的分离、鉴定及生物学特性研究[J].安徽农业科学,2011,39(18):11114-11118. |
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| 作者姓名： | 陈明 李健 廖洪敏 韦柳青 石云良 张为宇 黄维义 |
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| 作者单位： | 1. 广西大学动物科学技术学院,广西,南宁,530005;广西水产研究所,广西,南宁,530021
2. 广西大学动物科学技术学院,广西,南宁,530005 |
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| 基金项目： | 桂科技攻关项目(2011C06005) |
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| 摘 要： | 目的]弄清一株血液征状与鼠巴尔通氏体病相似的小鼠血源细菌的分类学地位及其与鼠巴尔通氏体的区别。方法]通过细菌常规鉴定及16SrRNA基因系统发育进化分析，对其进行了分类鉴定。利用免疫荧光和透射电镜观察、人工感染及免疫保护等试验，对该茵生物学特性进行研究。结果]常规分类鉴定显示分离到的细菌与丹毒科丹毒属细菌靠近，但其培养、染色及生化特性与该属的猪丹毒杆菌有较大差异。16SrRNA基因序列与猪红斑丹毒丝菌相似性达98．4％，系统进化树分析该菌与猪红斑丹毒丝菌亲源关系最近。免疫荧光和透射电镜观察结果表明该茵寄生在红细胞内部，但红细胞表面分泌有该细菌抗原成分。该细菌可通过尾静脉注射、腹腔注射、口腔感染及接触感染致死小白鼠。结论]建议将该细菌归为BXⅢ门（Firmicutes）Ⅱ纲（Mollicutes）V目（未定）I科（Erysipelotrichace—ae）I属（Erysipelothrix）的一新种（Erysipelothrixmuris sp．nou）。
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| 关 键 词： | 小白鼠 猪红宽丹毒丝茵 红细胞 16SrRNA基因 |
Isolation,Identification and Biological Characteristics Research of a Pathogen from the Blood of Mouse |
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| CHEN Ming et al.Isolation,Identification and Biological Characteristics Research of a Pathogen from the Blood of Mouse[J].Journal of Anhui Agricultural Sciences,2011,39(18):11114-11118. |
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| Authors: | CHEN Ming |
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| Institution: | CHEN Ming et al(Jinzhou Meteorology Bureau,Jinzhou,Liaoning 121001) |
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| Abstract: | By using measured meteorological data of Jinzhou during 1951-2010,the climatic variation characteristics of Jinzhou in recent 60 years were analyzed,as well as its influence to rural economic development,the corresponding countermeasures were also put forward.The results showed that precipitation is fluctuation,temperature and evaporation are increase gradually,the air becomes more dry,ground water levels decrease,which have a certain degree effect on rural economy sustainable development. |
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| Keywords: | Climate change Temperature Precipitation Rural economy Sustainable development |
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