| 绵羊卵母细胞蛋白质组2-DE图谱的构建及初步分析 |
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| 引用本文： | 林雍峰,张勇,李鸿岩,胡俊杰,赵兴绪.绵羊卵母细胞蛋白质组2-DE图谱的构建及初步分析[J].安徽农业科学,2011,39(16):9985-9987. |
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| 作者姓名： | 林雍峰 张勇 李鸿岩 胡俊杰 赵兴绪 |
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| 作者单位： | 甘肃农业大学动物医学院,甘肃,兰州,730070 |
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| 基金项目： | 科技部国际合作重点项目计划(2005DFA30720) |
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| 摘 要： | 目的]获得绵羊卵母细胞蛋白质组表达图谱。方法]采用直接裂解法制备卵母细胞蛋白样品,采用Bradford法和Ramagli法测定绵羊卵母细胞蛋白质样品的浓度,并进行双向凝胶电泳,运用PDQuest8.0对2-DE图谱进行初步分析。结果]Ramagli法能更准确地测定绵羊卵母细胞蛋白质样品的浓度,从而优化样品的上样量,得到更高质量的2-DE图谱;Bradford法测得的绵羊卵母细胞蛋白质浓度偏高。结论]700个卵母细胞的上样量比较合理,可用于构建卵母细胞蛋白质组2-DE图谱。
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| 关 键 词： | 绵羊 卵母细胞 双向电泳 蛋白质组 |
Construction and Preliminary Analysis of Ovine Oocytes Proteomic 2-DE Map |
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| LIN Yong-feng et al.Construction and Preliminary Analysis of Ovine Oocytes Proteomic 2-DE Map[J].Journal of Anhui Agricultural Sciences,2011,39(16):9985-9987. |
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| Authors: | LIN Yong-feng |
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| Institution: | LIN Yong-feng et al(College of Veterinary Medicine,Gansu Agricultural University,Lanzhou,Gansu 730070) |
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| Abstract: | Objective] To obtain ovine oocytes 2-dimensional gel electrophoresis(2-DE) maps.Method] Ovine oocytes were solubilized in lysis buffer,and protein concentrations were measured using the method of Bradford and Ramagli.Then the samples were subjected to two-dimensional polyacrylamide gel electrophoresis.The 2-DE maps of ovine oocytes were analyzed through PDQuest8.0.Result] The Ramagli method could reflect concentration of oocyte protein more actually and could optimize the quantity of samples to get a 2-D... |
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| Keywords: | Ovine Oocytes Two-dimensional polyacrylamide gel electrophoresis Proteome |
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