| 龙牙百合花氨基酸含量的柱前衍生OPA-HPLC法测定 |
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| 引用本文： | 陈立德,刘新桃,蒋盛岩,危兆安,张咋如.龙牙百合花氨基酸含量的柱前衍生OPA-HPLC法测定[J].安徽农业科学,2011,39(18):10832-10833,10836. |
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| 作者姓名： | 陈立德 刘新桃 蒋盛岩 危兆安 张咋如 |
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| 作者单位： | 邵阳学院生物与化学工程系,湖南,邵阳,422000;湖南省绿色食品办公室,湖南,长沙,410005;岳阳职业技术学院医学基础部,湖南,岳阳,414000 |
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| 基金项目： | 湖南省科技厅计划项目(2010NK3041) |
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| 摘 要： | 目的]测定龙牙百合花中的氨基酸含量。方法]以邻苯二甲醛（OPA）与9-芴基羰酰氯（FMOC—C1）作衍生剂，利用VWD检测器可变波长检测程序，采用柱前衍生OPA—HPLC法测定邵阳龙牙百合花中的17种氨基酸含量。结果]邵阳龙牙百合花中含有苏氨酸、丝氨酸、脯氨酸、蛋氨酸、谷氨酸、甘氨酸、亮氨酸、苯丙氨酸、酪氨酸、天门冬氨酸、丙氨酸、组氨酸、半胱氨酸、异亮氨酸等17种氨基酸。其中必需氨基酸的含量为49．7mg／g，占氨基酸总量的31．20％；儿童必需氨基酸的含量为30．6mg／g，占氨基酸总量的19．20％；含量最高的是谷氨酸（28．7mg／g，占氨基酸总量的18．03％）；其次是精氨酸（27．5mg／g，占氨基酸总量的17．30％）。结论]该方法操作简便，灵敏度高，结果准确，可靠。龙牙百合花中氨基酸的种类齐全，含量丰富，具有很高的开发利用价值。
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| 关 键 词： | 柱前衍生法 邻苯二甲醛衍生高效液相色谱法 百合花 氨基酸 |
Determination of Amino Acid in Flower of Lilium brownii var.viridulum by Reversed-phase High-Performance Liquid Chromatography with Pre-column Derivation |
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| CHEN Li-de et al.Determination of Amino Acid in Flower of Lilium brownii var.viridulum by Reversed-phase High-Performance Liquid Chromatography with Pre-column Derivation[J].Journal of Anhui Agricultural Sciences,2011,39(18):10832-10833,10836. |
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| Authors: | CHEN Li-de |
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| Institution: | CHEN Li-de et al(Department of Biological and Chemical Engineering,Shaoyang University,Shaoyang,Hunan 422000) |
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| Abstract: | Objective] To determine the contents of amino acids in flower of Lilium brownii var.viridulum.Method] Contents of 17 amino acids in flower of Lilium brownii var.viridulum were determined using OPA-HPLC method,in which o-phthalaldehyde(OPA) and 9-fluore-nylmetloy chorofomnate(FMOC) were selected as pre-column derivative reagent and VWD detector was used.Result] The Lilium brownii var.viridulum from Shaoyang was rich in Asp,Glu,Ser,His,Gly,Thr,Arg,Ala,Tyr,Cys,Val,Met,Phe,Iso,Leu,Lys and Pro.The contents of... |
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| Keywords: | Pre-column derivation OPA-HPLC Lilium brownii var viridulum Amino acid |
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