| 广西地区甘蔗黄叶病病毒分子鉴定 |
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| 引用本文： | 颜梅新,黄伟华,黄诚华.广西地区甘蔗黄叶病病毒分子鉴定[J].安徽农业科学,2011(17):10156+10163-10156,10163. |
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| 作者姓名： | 颜梅新 黄伟华 黄诚华 |
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| 作者单位： | 广西甘蔗研究所广西甘蔗遗传改良重点实验室; |
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| 基金项目： | 现代农业产业技术体系建设专项资金项目(nycytx-024-01-07); 广西自然科学基金项目(2010GXNSFB013026); 广西农业科学院基本科研专项(G2010010,201004) |
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| 摘 要： | 目的]为广西甘蔗黄叶综合征（YLS）的分子检测和抗病育种提供参考。方法]采集显症甘蔗叶样本，通过提取总RNA，利用引物P1／P2对样本递行反转录RCR（RT-PCR）扩增，对广西地区甘蔗黄叶病的病原体进行分子鉴定。结果]经引物P1／P2扩增出630 bp的目的片段。氨基酸和核苷酸序列分析结果表明，该片段是甘蔗黄叶病毒（SCYLV）外壳蛋白基因的一部分，与其他国家SCYLV分离物同源性达95％-99％。结论]广西甘蔗黄叶病病原确是SCYLV，该病毒可能是近年从区外或境外传入的。
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| 关 键 词： | 甘蔗 黄叶病 甘蔗黄叶病毒 分子鉴定 |
Molecular Identification of Sugarcane Yellow Leaf Virus in Guangxi |
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| YAN Mei-xin et al.Molecular Identification of Sugarcane Yellow Leaf Virus in Guangxi[J].Journal of Anhui Agricultural Sciences,2011(17):10156+10163-10156,10163. |
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| Authors: | YAN Mei-xin |
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| Institution: | YAN Mei-xin et al (Guangxi Sugarcane Research Institute,Guangxi Key Laboratory of Sugarcane Genetic Improvement Nanning,Guangxi 530007) |
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| Abstract: | Objective] The reference for the molecular detection of sugarcane yellow leaf(YSL) and the disease-resisting breeding of sugarcane in Guangxi was provided through the research. Method] Total RNA, extracted from the sample with the disease symptom, was amplified with specific primers P1/P2 for RT-PCR. Result] A distinct fragment of about 630 bp was amplified. The analysis of the nucleotide sequence of the RT-PCR product was found it was the part the SCYLV, which was 95%-99% identical to sequence of the SC... |
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| Keywords: | Sugarcane Yellow leaf disease Sugarcane yellow leaf virus Molecular identification |
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