| 几株Bt菌株对紫外线的抗性研究 |
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| 引用本文： | 许会才,王卫国,赵永亮.几株Bt菌株对紫外线的抗性研究[J].安徽农业科学,2008,36(8):3086-3087. |
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| 作者姓名： | 许会才 王卫国 赵永亮 |
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| 作者单位： | 焦作师范高等专科学校,河南,焦作,454000;河南工业大学生物工程学院,河南,郑州,450001 |
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| 基金项目： | 河南省教育厅自然科学基金资助项目(No.9711268)部分内容. |
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| 摘 要： | 目的]寻求高抗紫外线的Bt菌株。方法]采用不同照射时间的紫外线(UV)对1株野生型Bt菌株及3株Bt菌株Bt001、Bt200、Bt087进行照射处理后再置于32℃培养箱中培养16h。结果]Bt野生菌株在UV处理3min后已基本全部失活,而Bt001、Bt200、Bt087在UV处理8min后仍有活性。菌株Bt001、Bt200、Bt087对UV的抗性均强于野生Bt菌株,其中,Bt200相对弱些,UV处理9min后已无菌落;Bt087最弱,UV处理8min后已无菌落;而菌株Bt001对UV的抗性最强,UV处理13min后仍有菌落出现,说明不同的Bt菌株,其遗传背景是不同的。另外,Bt001经紫外线照射9min培养16h后发现有9个菌落发生明显的变异,菌落呈棕黄色,菌落明显比原始菌落小,油镜下观察涂片,其菌体比原始菌体小,说明Bt001为明显发生突变的菌株。结论]菌株Bt001对UV的抗性最强。
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| 关 键 词： | 苏云金芽孢杆菌 抗紫外线性 分离 |
| 文章编号： | 0517-6611(2008)08-03086-02 |
| 修稿时间： | 2007年12月21 |
Discussion on the Resistances of Several Bt Strains to UV Radiation |
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| XU Hui-cai.Discussion on the Resistances of Several Bt Strains to UV Radiation[J].Journal of Anhui Agricultural Sciences,2008,36(8):3086-3087. |
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| Authors: | XU Hui-cai |
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| Abstract: | Objective]The purpose was toseek for Bt strains with high resistance to UV radiation. Method] A wild Bt strain and 3 Bt strains of Bt001, Bt200, Bt087 were radiated by UV radiation for different time, and then cultured in incubator at 32 ℃for 16 h. Result] Almost all the wild Bt strains lost their activities after they were treated by UV radiation for 3 min, but Bt001, Bt200 and Bt087 also had activities after they were treated by UV radiation for 8 min. The resistances of strains Bt001, Bt200 and Bt087 to UV radiation were stronger than that of the wild Bt strain, among which, Bt200 was relatively weaker, there was no colony after it was treated by UV radiation for 9 min; Bt087 was weakest, there was nocolonyafter it was treated byUVradiation for 8 min; the resistance ofthe strain Bt001 toUVradiation was strongest, there was alsocolony appeared after it was treated by UV radiation for 13 min, indicating that the genetic backgrounds of different Bt strains were different. Besides, after Bt001 was treated by UV radiation for 9 min and then cultured for 16 h, there were 9 colonies of Bt001 found to have significant variations and they were brown yellowand significantly smaller than their initial colonies. It was found that their cells were smaller than their initial cells through observingtheir smears under oil microscope, indicatingthat Bt001 was the strain with significant variation. Conclusion]The resistance of the strain Bt001 toUVradiation was strongest. |
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| Keywords: | Bacillus thuringiensis Resistance toultraviolet radiation Separation |
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