| 兰花种子的原球茎诱导及其生长分化研究 |
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| 引用本文： | 古碧珠,刘柏涛,何嘉碧,梁红.兰花种子的原球茎诱导及其生长分化研究[J].安徽农业科学,2008,36(31). |
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| 作者姓名： | 古碧珠 刘柏涛 何嘉碧 梁红 |
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| 作者单位： | 仲恺农业工程学院生命科学学院,广东广州,510225;仲恺农业工程学院生命科学学院,广东广州,510225;仲恺农业工程学院生命科学学院,广东广州,510225;仲恺农业工程学院生命科学学院,广东广州,510225 |
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| 摘 要： | 目的]从培养基方面优化兰花快速繁殖的技术体系。方法]将石斛兰和秋花独蒜兰的干种子培养于MS、B5、KC液体培养基上,诱导原球茎形成,再将原球茎培养于MS、KC及B5固体培养基中,研究原球茎分化苗在不同培养基上的生长发育情况。结果]石斛兰和秋花独蒜兰种子在B5液体培养基中诱导产生的原球茎最多,萌发率分别为3.1%、7.3%。秋花独蒜兰原球茎在KC固体培养基上的成活率最高,为71.9%,且幼苗生长发育状况最好,原球茎直径为(5.8±0.3)mm;石斛兰原球茎在B5固体培养基上的成活率最高,为65.0%,在KC固体培养基上的生长发育状况最好,原球茎直径为(3.8±0.6)mm。结论]根据不同的组培快繁目标,可在兰花的不同生长发育阶段选用合适的培养基。
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| 关 键 词： | 石斛兰 秋花独蒜兰 原球茎 培养基 |
Study on Induction of Protocorms from Cymbidium Seeds and Its Growth and Differentiation |
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| Abstract: | Objective] The aim was to optimize the technical system for the rapid propagation of cymbidium from aspect of the medium.Method]The dry seeds of Dendrobium nobile and Pleione maculata were cultured on MS,B5 and KC liquid media for inducing protocorms,and the protocorms were subcultured on MS,KC and B5 solid media to study the growth of protocorm differentiation seedlings in the different medium.Result] The protocorms of D.nobile and P.maculata seeds induced in the B5 liquid medium were most and their germination rate were 3.1% and 7.3%,resp.The survival rate of P.maculate protocorm was highest in the KC solid medium,being 71.9%,and their seedling growth was best and the diameter of the protocorm was(5.8±0.3) mm.The survival rate of D.nobile protocorm was highest in the B5 solid medium,being 65.0%,and their seedling growth was best in the KC solid medium and the diameter of the protocorm was(3.8±0.6) mm.Conclusion] The suitable medium could be selected in the different developmental stages of cymbidium according to the different goals of tissue culture and rapid propagation. |
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| Keywords: | Dendrobium nobile Pleione maculate Protocorm Medium |
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