| 奥利亚罗非鱼(♀)×鳜(♂)杂交后代及其双亲的染色体核型分析 |
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| 引用本文： | 曹丽萍,丁炜东,贾永义,夏德全,吴婷婷.奥利亚罗非鱼(♀)×鳜(♂)杂交后代及其双亲的染色体核型分析[J].农业生物技术学报,2006,14(2):187-190. |
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| 作者姓名： | 曹丽萍 丁炜东 贾永义 夏德全 吴婷婷 |
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| 作者单位： | 中国水产科学院淡水渔业研究中心,无锡,214081 |
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| 基金项目： | 中国科学院资助项目;国家科技攻关项目;国家科技攻关项目 |
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| 摘 要： | 采用人工授精法以奥利亚罗非鱼(Oreochromis aurea)为母本，鳜(Siniperca ahuasti)为父本，获得了能正常发育的后代。利用微量全血培养法对奥利亚罗非鱼、鳜及它们杂交后代的染色体数目和核型进行了分析，结果表明, 奥利亚罗非鱼染色体数目为2n =44，核型公式为 6sm+24st+14t，染色体臂数(NF)=50；鳜鱼为2n =48，核型公式为 8sm+4st+36t，NF=56；杂交后代为2n =44，核型公式为 6sm+26st+12t，NF=50。经对比分析，杂交后代与奥利亚罗非鱼的核型相似。
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| 关 键 词： | 奥利亚罗非鱼 鳜 远缘杂交 染色体 |
| 文章编号： | 1006-1304(2006)02-0187-04 |
| 收稿时间： | 2005-4-6 |
| 修稿时间： | 2005年3月30日 |
Analysis of Chromosome Karyotypes of Oreochromis aurea(♀), Siniperca chuatsi(♂) and Their Offspring |
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| CAO Li-ping,DING Wei-dong,JIA Yong-yi,XIA De-quan,WU Ting-ting.Analysis of Chromosome Karyotypes of Oreochromis aurea(♀), Siniperca chuatsi(♂) and Their Offspring[J].Journal of Agricultural Biotechnology,2006,14(2):187-190. |
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| Authors: | CAO Li-ping DING Wei-dong JIA Yong-yi XIA De-quan WU Ting-ting |
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| Abstract: | The progenies of the distant hybridization between Oreochromis aurea (♀) and Siniperca ahuasti (♂) were successfully obtained through years of research. And their karyotypes were studied by half-micro blood lymphocytic cell cultivation. The result indicated that the diploid chromosome number of O. aurea (♀) was 2n =44, and karyotype formula was 6sm+24st+14t, arm number (NF) =50; the diploid chromosome number of S. chuatsi (♂) was 2n =48, and karyotype formula was 8sm+4st+36t, NF=56; the diploid chromosome number of their progenies was 2n =44, chromosome formula was 6sm+26st+12t, NF=50. The results showed the karyotypes between O. aurea (♀) and progenies were similar by comparison. |
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| Keywords: | Oreochromis aurea Siniperca chuatsi distant hybridization chromosome |
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