Intrinsic innervation of the horse ileum |
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| Authors: | Roberto Chiocchetti Cristiano Bombardi Caterina Mongardi-Fantaguzzi Elena Venturelli Domenico Russo Alessandro Spadari Corrado Montoneri Noemi Romagnoli Annamaria Grandis |
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| Institution: | aDepartment of Veterinary Morphophysiology and Animal Productions, University of Bologna, 40064 Ozzano Emilia, Bologna, Italy;bVeterinary Clinical Department, University of Bologna, 40064 Ozzano Emilia, Bologna, Italy |
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| Abstract: | This paper describes the morphology and distribution of the enteric nervous system (ENS) cells and fibres immunoreactive for choline acetyltransferase (ChAT), neuronal nitric oxide synthase (nNOS), substance P (SP), calcitonin gene-related peptide (CGRP), NF200 kDa (NF200), and S100 protein. The percentages of subclasses of enteric neurons in the total neuronal population were investigated by the use of anti-PGP 9.5 or anti-NSE antibodies.ChAT-IR myenteric plexus (MP) and submucosal plexus (SMP) neurons were 66 ± 7% and 74 ± 15%, respectively, whereas those cells expressing nNOS-IR were 38 ± 7% and 5 ± 1%, respectively. MP and SMP neurons expressing both phenotypes were also present. SP-IR was expressed by 14 ± 13% of MP and 66 ± 8% of SMP neurons whereas CGRP-IR was observed only in the SMP (43 ± 6%). NF200-IR was expressed by 61 ± 15% and 91 ± 10% of the MP and SMP neurons, respectively. The majority of the CGRP-IR SMP neurons expressed also SP-IR. Almost all SP-IR neurons in both the plexuses were cholinergic. The present study quantifies the main neuronal subpopulations of the ENS of the horse ileum; these data might be utilized to understand the neuronal modifications which occur in several gastrointestinal tract disorders. |
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| Keywords: | Horse Ileum Enteric nervous system Immunohistochemistry |
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