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| 巴西橡胶树HbMT2植物表达载体的构建及遗传转化烟草 | |
| 引用本文： | 吴瑞,朱家红,梁黔云.巴西橡胶树HbMT2植物表达载体的构建及遗传转化烟草[J].广东农业科学,2013,40(6):138-142. |
| 作者姓名： | 吴瑞 朱家红 梁黔云 |
| 作者单位： | 1. 毕节市农业科学研究所,贵州毕节551700;中国热带农业科学院热带生物技术研究所,海南海口570100 2. 中国热带农业科学院热带生物技术研究所,海南海口,570100 3. 毕节市农业科学研究所,贵州毕节,551700 |
| 基金项目： | 天然橡胶产业技术体系基金，国家自然科学基金 |
| 摘 要： | 将巴西橡胶树金属硫蛋白基因HbMT2插入到植物表达载体pCAMBIA 1304,得到HbMT2基因的植物表达载体pCAMBIA1304-HbMT2.通过电击法将该载体导入根癌农杆菌菌株中并转化烟草.将获得的具有潮霉素(Hyg)抗性的再生植株进行PCR鉴定.结果表明目的基因已经整合到烟草基因组中.抗逆性分析试验显示,转基因烟草对H202处理和紫外线照射耐受性显著提高；重金属离子胁迫下,转基因烟草叶绿素含量、过氧化物酶和过氧化氢酶活性明显高于对照. |
| 关 键 词： | 巴西橡胶树 金属硫蛋白 载体构建 遗传转化 抗逆性分析 |
Construction of plant expression vector for the HbMT2 gene of Hevea brasiliensis and its genetic transformation tobacco |
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| WU Rui , ZHU Jia-hong , LIANG Qian-yun.Construction of plant expression vector for the HbMT2 gene of Hevea brasiliensis and its genetic transformation tobacco[J].Guangdong Agricultural Sciences,2013,40(6):138-142. | |
| Authors: | WU Rui ZHU Jia-hong LIANG Qian-yun |
| Abstract: | The HbMT2 was constructed into pCAMBIA1304 expression vector, and transformed into tobacco (Nicotiana tabacum) mediated by Agrobacterium, Hygromycin B (Hyg) resistant regenerated plantlets of tobacco were selected. PCR detection indicated that all the Hyg resistant tobacco plants contained the alien HbMT2 gene. Resistance analysis tests showed that the tolerance of transgenic plants to hydrogen peroxide and ultraviolet irradiation was significantly increased, and the chlorophyll content and the activity of peroxidase and catalase were significantly higher in trangenic plants than the controls in heavy-metal stress condition. |
| Keywords: | Hevea brasiliensis metallothionein(MT) construction of expression genetic transformation resistance analysis |
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