| 边缘细胞对大豆根尖铝毒害的缓解 |
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| 引用本文： | 李荣峰,蔡妙珍,刘鹏,徐根娣,梁和,周主贵.边缘细胞对大豆根尖铝毒害的缓解[J].作物学报,2008,34(2):318-325. |
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| 作者姓名： | 李荣峰 蔡妙珍 刘鹏 徐根娣 梁和 周主贵 |
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| 作者单位： | 1 浙江师范大学植物学重点实验室, 浙江金华321004; 2 广西大学农学院, 广西南宁530005 |
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| 基金项目： | 国家科技攻关项目
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国家自然科学基金
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浙江省自然科学基金 |
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| 摘 要： | 以大豆Glycine max (L.) Merrill]浙春3号为试验材料, 采用静置培养(保持边缘细胞附于根尖)和振荡培养(移除根尖边缘细胞), 测定边缘细胞数目和活性、根相对伸长率和根内酶的活性, 研究了边缘细胞对大豆根尖铝毒害的缓解效应。结果显示, 大豆发育过程中存活的边缘细胞数与总数之比达60%~80%, 50~400 mmol L-1 Al3+胁迫12 h能诱导边缘细胞从根尖脱落, 200~400 μmol L-1 Al3+胁迫24 h对边缘细胞的发育有抑制作用。Al3+处理抑制根伸长, 移除边缘细胞的根相对伸长率低于保留边缘细胞的根。0~100 mmol L-1 Al3+胁迫12 h, 0和50 mmol L-1Al3+胁迫24 h, 具有边缘细胞的大豆根系的POD、SOD活性及蛋白质含量显著高于移除边缘细胞的根内酶活性和蛋白质含量, 但200和400 mmol L-1 Al3+处理12 h, 100~400 mmol L-1 Al3+处理24 h时, 根尖有无边缘细胞对根系的酶活性及蛋白质含量影响不显著。说明低浓度Al3+胁迫下, 大豆通过增加边缘细胞数目、提高根尖蛋白质含量, 维持较高水平的POD、CAT和SOD活性来对抗铝毒胁迫, 以缓解植物的铝毒害。
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| 关 键 词： | 边缘细胞 大豆 铝毒 缓解效应 |
| 收稿时间： | 2006-12-04 |
| 修稿时间： | 2007-06-12 |
Border Cells Alleviating Aluminum Toxicity in Soybean Root Tips |
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| LI Rong-Feng,CAI Miao-Zhen,LIU Peng,XU Gen-Di,LIANG He,ZHOU Zhu-Gui.Border Cells Alleviating Aluminum Toxicity in Soybean Root Tips[J].Acta Agronomica Sinica,2008,34(2):318-325. |
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| Authors: | LI Rong-Feng CAI Miao-Zhen LIU Peng XU Gen-Di LIANG He ZHOU Zhu-Gui |
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| Institution: | 1.Key Laboratory of Botany, Zhejiang Normal University, Jinhua 321004, Zhejiang;2.Agricultural College of Guangxi University, Nanning 530005, Guangxi, China |
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| Abstract: | Root border cell (BC) is a group of cells from the apex of root, which has been considered to play a vital role in protecting root tip from extracellular biotic and abiotic stresses. It is known that the BC may secrete saccharide to chelate Al3+ and alleviate cell damage under aluminum stress. In this paper, we aimed to reveal the effects of root BC on alleviation of aluminum toxicity to root tips with soybean Glycine max (L.) Merrill] cultivar Zhechun 3 during the development of roots. The BC number, activities of peroxidase (POD), catalase (CAT), and superoxide dismutase (SOD) as well as protein content were measured under 0 (CK), 50, 100, 200, and 400 μmol L-1 Al3+ stress for 12 and 24 h respectively, with static culture (to maintain border cells adhered to root tips) and shaking culture (to remove border cells from root tips completely). The results showed that the viable border cells accounted for 60%–80% when root length was 5 mm. 50–400 mmol L-1 Al3+ treatment for 12 h increased the number of border cells, but inhibition of border cell development by Al3+ turned up after 200, 400 mmol L-1 Al3+ treatment for 24 h. When soybean roots were cultivated by shaking to remove border cells from root tips, root elongation was decreased with increasing Al3+ concentrations. However, this kind of inhibition of root growth was alleviated under static culture. At the same time, POD, CAT (under Al stress for 12 h), SOD activities and protein content were higher significantly than those under shaking culture withlower Al3+ concentration (0–100 mmol L-1 Al3+ treatment for 12 h and 0, 50 mmol L-1 Al3+ treatment for 24 h) stress. And no significant difference in POD, SOD activities and protein content was observed between static culture and shaking culture under higher Al3+ concentration (200, 400 mmol L-1 Al3+ treatment for 12 h and 100–400 mmol L-1 Al3+ treatment for 24 h) (P>0.05). The results suggested that border cells adhered to the root tips can significantly affect root relative elongation rate, POD, CAT, SOD activities and protein content of soybean root tip, and alleviate the toxicity Al3+ to plant root tips through increasing the number of border cells, enhancing the protein content of root tips and maintaining higher POD and SOD activities. |
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| Keywords: | Border cell Soybean Aluminum toxicity Alleviating effect |
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