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| 塔里木马鹿微卫星遗传多样性与产茸量的相关性研究 | |
| 引用本文： | 袁国伟,席继峰,郭庆河,袁其彬,钟灵,彭林泽,曾献存,贾斌.塔里木马鹿微卫星遗传多样性与产茸量的相关性研究[J].经济动物学报,2008,12(1):6-13. |
| 作者姓名： | 袁国伟 席继峰 郭庆河 袁其彬 钟灵 彭林泽 曾献存 贾斌 |
| 作者单位： | 1. 石河子大学动物科技学院,石河子,832003;乌鲁木齐华农科技有限公司,乌鲁木齐830000 2. 新疆农业职业技术学院动物科学系,乌鲁木齐,831100 3. 新疆阿拉尔南口农场林牧科,阿克苏,843000 4. 新疆阿克苏5团兽医站,阿克苏,843015 5. 石河子大学动物科技学院,石河子,832003 |
| 基金项目： | 石河子大学自然基金项目资助课题(ZRKX2004012) |
| 摘 要： | 利用7个微卫星遗传标记,采用PCR扩增,12%非变性聚丙烯酰胺凝胶电泳、Sanguinetti银染法显色,对新疆塔里木马鹿亚种遗传多样性进行检测,统计各群体的等位基因组成、平均有效等位基因数(E)和平均基因纯合率(Rh),根据等位基因频率计算出各群体的平均遗传杂合度(h)、多态信息含量(PIC)和群体间的遗传距离,利用分子进化遗传分析软件(MEGA),采用邻结法(NJ)重建系统发生树。根据等位基因频率,利用PHYLIP(3.6)分析软件,采用最大似然法(ML)构建系统发生树,应用自举检验(bootstrap test)估计系统树中节点的自引导值(bootstrap value),并进行了系统发生分析。同时对部分马鹿群体个体基因型与产茸量之间进行了相关分析。结果表明:7个微卫星位点在3个塔里木马鹿中的多态信息含量除BMS2508和Celjp0023没有多态性,以及BM5004为中度多态外,其他4个微卫星均为高度多态,可作为有效的遗传标记用于3个塔里木马鹿群体遗传多样性和系统发生关系的分析。所有马鹿群体的平均PIC为(0.5196)、h(0.5552)和E(2.45),其基因多态性和遗传多样性相对丰富。塔里木马鹿35团群体与沙雅群体的血缘关系及遗传距离近于阿拉尔群体,3个塔里木马鹿群体的系统发育关系基本符合其地理分布和育成史。微卫星位点BM4208的166bp/185bp基因型和微卫星BM888的208bp/208bp基因型可以对塔里木马鹿35团群体高产茸量进行分子遗传标记。 |
| 关 键 词： | 马鹿 微卫星 遗传多样性 |
| 文章编号： | 1007-7448(2008)01-0006-07 |
| 修稿时间： | 2007年8月8日 |
Relationships between Genetic Diversity of Microsatellites and Anter Production Capacity of Tarim Red Deer |
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| YUAN Guo-wei,XI Ji-feng,GUO Qing-he,YUAN Qi-bin,ZHONG Ling,PENG Lin-ze,ZENG Xian-cun,JIA Bin.Relationships between Genetic Diversity of Microsatellites and Anter Production Capacity of Tarim Red Deer[J].Journal of Economic Animal,2008,12(1):6-13. | |
| Authors: | YUAN Guo-wei XI Ji-feng GUO Qing-he YUAN Qi-bin ZHONG Ling PENG Lin-ze ZENG Xian-cun JIA Bin |
| Abstract: | The genetic polymorphism of seven microsatellite loci of Xinjiang Tarim red deer was detected by polymerase chain reaction(PCR),12% nondenaturation polyacrylamide gel electrophoresis and Sanguinetti silver staining method. Numbers of alleles,average effective numbers of alleles(E) and average rate of homozygote of three subspecies populations were counted.According to allelic frequencies of seven microsatellite loci polymorphism information content(PIC),mean heterozygosity(h) and genetic distance among the populations were calculated for each population.The dendrograms was obtained based on genetic distance by Neighbor-join method(NJ),utilizing Molecular Evolutionary Genetics Analysis soft(MEGA).At the same time,the phylogenetic tree was constructed based on allelic frequencies by Maximum likelihood(ML),the bootstrap value was estimated by bootstrap test in the tree,lastly,phylogenetic relationships was analyzed.The relations between genetic markers and production capacity of antler was analyzed.The results suggests that four of seven microsatellite loci are high polymorphic,but BMS2508 and Celjp0023 have no polymorphism and BM5004 is neutral polymorphism.It is the conclusions that the four microsatellite loci are effective DNA markers for analysis of genetic diversity and phylogenetic relationship among three Tarim red deer populations.The mean PIC,h and E of the whole microsatellite loci is 0.519 6,0.555 2 and 2.45,which shows that these microsatellite loci are effective DNA markers for the genetic analysis of red deer.The phylogenetic relationship among different red deer populations is consistent with history of breeding and distribution of geography.The genetic distance and consanguinity between REGIMENT 35 population and XAYA population is closer than that between REGIMENT 35 and ALAER population.The phylogenetic relationship among different red deer populations is consistent with history of breeding and distribution of geography.The genotypes of 166bp/185bp in BM4208 locus and 208bp/208bp in BM888 locus are useful markers for production capacity of antler in REGIMENT 35 population. |
| Keywords: | red deer microsatellite genetic diversity |
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