| 家养野猪副猪嗜血杆菌的分离鉴定及16S rRNA序列分析 |
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| 引用本文： | 肖根辉,王萍,宋德平,花象柏,何后军,邬向东,邓舜洲,黎家祥.家养野猪副猪嗜血杆菌的分离鉴定及16S rRNA序列分析[J].兽医大学学报,2012(7):955-957,966. |
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| 作者姓名： | 肖根辉 王萍 宋德平 花象柏 何后军 邬向东 邓舜洲 黎家祥 |
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| 作者单位： | [1]江西农业大学动物科技学院,江西南昌330045 [2]南昌珠安生物技术有限公司,江西南昌330013 |
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| 摘 要： | 对江西省某家养野猪场临诊疑似副猪嗜血杆菌（Haemophilusparasuis，Hps）感染的病例进行细菌分离鉴定，PCR扩增分离菌株的16SrRNA并进行测序分析，并对分离菌进行细菌形态、生化鉴定和PCR鉴定及序列比对分析。结果显示，获得1株家养野猪源Hps分离株（命名为HPJXYZ01），该分离株与国内外参考菌株序列之闻的同源性为93．1％～99．2％，与本实验室江西省家猪源分离株的同源性为84％～92．1％。结果表明，江西省家养野猪中存在Hps感染，分离株与国内外家猪源Hps间的16SrRNA序列差异不大，Hps16SrRNA核苷酸序列比较稳定，其进化不存在明显的地域相关性。
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| 关 键 词： | 家养野猪 Hps 分离鉴定 16S rRNA 序列分析 |
Isolation and identification of Haemophilus parasuis and analysis of 16S rRNA gene |
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| Authors: | XIAO Gen-hui WANG Ping ＂ SONG De-pingI HUA Xiang-bo HE Hou-jun WU Xiang-dong DENG Shun-zhoua LI Jia-xiang |
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| Institution: | 1. College of Animal Science and Technology, J iangxi Agri- cultural University, Nanchang 330045, China 2. Nanchang Zhuan Biotechnology Co, Ltd. Nan- chang 330013 ,China) |
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| Abstract: | Isolated and identified the bacteria from the suspected Haemophilus parasuis （Hps） infection cases from one domesticated wild boar farm in Jiangxi province, amplified 16S rRNA of the strains and sequenced. The strain was identified based on bacterial morphology, biochemical identification,PCR identification and sequence analysis, one strain was isolated from the domesticated wild boar and named HPJXYZO1. The sequences homology of the isolation with reference strains was ranged from 93.1% to 99.2 %, and was between 84 %--92.1~ with other two Jiangxi i- solates from our laboratory. Hps could infect the domesticated wild boar from Jiangxi province, and there is little difference in the 16S rRNA sequence between reference strains arid the isolate, the Hps 16S rRNA nucleotide se- quence is relatively stable,and there is not obvious geographical correlation in evolution. |
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| Keywords: | domesticated wild boar Hps isolation and identification 16 S rRNA sequence analysis |
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