| 腺病毒表达鸡GM-CSF及其对鸡骨髓细胞增殖活性的检测 |
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| 引用本文： | 王兴龙,郝华芳,杜恩岐,唐文雅,张鹏,陈胜利,张渭东,张淑霞,党如意,杨增岐.腺病毒表达鸡GM-CSF及其对鸡骨髓细胞增殖活性的检测[J].兽医大学学报,2012(4):509-512. |
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| 作者姓名： | 王兴龙 郝华芳 杜恩岐 唐文雅 张鹏 陈胜利 张渭东 张淑霞 党如意 杨增岐 |
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| 作者单位： | 西北农林科技大学动物医学院,陕西杨凌712100 |
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| 基金项目： | “十一五”国家科技支撑计划资助项目（2007-2010） |
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| 摘 要： | 为了方便鸡粒细胞/巨噬细胞集落细胞刺激因子（GM-CSF）作为疫苗佐剂使用,用PCR方法扩增出鸡GM-CSF基因,将其连入人腺病毒穿梭载体pAdTrack-CMV,将重组穿梭载体与腺病毒骨架重组后,获得重组腺病毒骨架载体,线性化后转染293SD细胞,获得能够表达鸡GM-CSF的腺病毒。将获得的重组腺病毒转导鸡成纤维细胞系DF1,细胞培养上清中,可以检测到GM-CSF刺激鸡骨髓细胞活性,为鸡GM-CSF进一步应用奠定了基础。
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| 关 键 词： | 鸡GM-CSF 重组腺病毒 活性检测 |
Adenovirus expression and activity test of chicken GM-CSF |
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| Authors: | WANG Xing-long HAO Hua-fang DU En-qi TANG Wen-ya ZHANG Peng CHEN Sheng-li ZHANG Wei-dong ZHANG Shu-xia DANG Ru-yi YANG Zeng-qi |
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| Institution: | (College of Veterinary Medicine,Northwest A＆F University,Yangling,Shaanxi 712100,China) |
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| Abstract: | To conveniently use chicken GM-CSF as cytokines adjuvant in chicken vaccine,chicken GM-CSF were amplified and cloned into adenovirus shuttle vector.After recombinant with the linearization shuttle vector,the recombinant plasmid containing chicken GM-CSF was used to transfect 293SD cells.The recombinant adenovirus containing chicken GM-CSF was recovered from the 293SD cells.The recombinant adenovirus were transducted into chick embryo fibroblast cells（cell line DF1）,in the culture medium,the activity of chicken GM-CSF could be detected.The recombinant adenovirus may be a useful candidate for improve chicken vaccine effect. |
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| Keywords: | chicken GM-CSF recombinant adenovirus activity |
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