| 不同物种NF-κB1基因编码区生物信息分析 |
| |
| 引用本文： | 张军杰,李祥龙,周荣艳,李兰会,任玉红,赵驻军.不同物种NF-κB1基因编码区生物信息分析[J].兽医大学学报,2012(9):1404-1408,1412. |
| |
| 作者姓名： | 张军杰 李祥龙 周荣艳 李兰会 任玉红 赵驻军 |
| |
| 作者单位： | 河北农业大学动物科技学院,河北保定071000 |
| |
| 基金项目： | 国家自然科学基金资助项目（31172196） |
| |
| 摘 要： | 为了解不同物种NF-κB1基因编码区（CDS）的遗传变异,本试验使用生物信息学的方法比较分析了小家鼠、褐家鼠、人、黑猩猩、狨、毛猩猩、猕猴、家马、大熊猫、野猪、家短尾负鼠、牛、狼、非洲象和白颊长臂猿NF-κB1基因编码区的遗传多样性,并对该基因的氨基酸序列、跨膜结构域、导肽、信号肽和结构域进行了预测和分析,对有关NF-κB1基因功能的一些研究热点进行了回顾。结果表明：在15个物种36条基因序列中共检测到968个多态位点,生成24种单倍型,NF-κB1基因序列编码区在物种间存在丰富的遗传多样性;理论等电点均低于5.5,NF-κB1编码的蛋白呈酸性,N端无信号肽、导肽,无跨膜结构域,肽链表现为亲水性;这些蛋白均含有1个Rel同源域,6～7个Ankyrin重复,在结构上保守。
|
| 关 键 词： | 物种 NF-κB1基因 生物信息学 |
Bioinformatics analysis of coding regions of NF-KB1 gene among species |
| |
| Authors: | ZHANG Jun-jie LI Xiang-long ZHOU Rong-yan LI Lan-hui REN Yu-hong ZHAO Zhu-jun |
| |
| Institution: | (College of Animal Science and Technology, Hebei Agricultural University, Baoding, Hebei 071000, China) |
| |
| Abstract: | In order to study the genetic variation of the NF-kB 1 gene CDS in different species, the genetic diversity of the CDS of NF-xB 1 gene sequences from Mus musculus, Rattus norvegicus, Homo sapiens, Pan troglodytes, Calithrix jacchus,Pongo abelii, Macaca mulatta, Equus caballus, Ailuropoda melanoleuca, Sus scrofa, Monodelphis domestica, Bos Taurus,Canis lupus,Loxodonta Africana and Nomascus leucogenys was analyzed using the method of bioinfor- matics. The characteristics of composition of nucleic acid sequences and amino acid sequences,leader peptides, signal peptide,trans-membrane to pological structure and structure domain was also analyzed, and some of the loci of intense investigation on the function of NF-kB1 were reviewed. The results showed that a total of 968 polymorphic sites were detected from 36 sequences of 15 species,from which 24 hapolotypes were sorted. The genetic diversity of the NF-kB1 gene CDS was rich among species. The NF-xB 1 was a non trans-membrane protein without leader pep- tides and signal peptide in N-terminal,and the polypeptide chain presented hydrophilic. These proteins contain a Rel homology domain and 6-7 Ankyrin repeat,conservative in structure. |
| |
| Keywords: | species NF-xB 1 genes bioinformatics |
| 本文献已被 维普 等数据库收录！ |
|
