| 梨果实中肉桂醇脱氢酶基因的克隆与序列分析 |
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| 引用本文： | 陶书田,赵梅,张彪,张绍铃.梨果实中肉桂醇脱氢酶基因的克隆与序列分析[J].中国南方果树,2012,41(3):40-45. |
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| 作者姓名： | 陶书田 赵梅 张彪 张绍铃 |
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| 作者单位： | 1. 南京农业大学梨工程技术研究中心,南京,210095
2. Horticultural Research and Development Centre, Agriculture and Agri-Food Canada |
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| 基金项目： | 国家自然科学基金，现代农业产业技术体系建设专项资金，江苏省博士后科研基金 |
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| 摘 要： | 肉桂醇脱氢酶(CAD)是木质素生物合成过程中的一个关键酶。本研究分别提取了"砀山酥梨"及"幸水"梨果肉总RNA,并通过RT-PCR、克隆和测序,成功获得了2个CAD的大小为689bp的cDNA片段,并分别命名为PB-CAD(登录号:FJ478151)和PP-CAD(登录号:FJ478152)。在核苷酸水平上,这两个基因片段只有4个碱基的差异,编码相似性达99.6%的两个氨基酸序列,该序列由229个残基组成。通过蛋白质序列比较,发现梨果实CAD与许多植物中CAD氨基酸序列极为相似,与苹果Malus domestica(AAC06319)、梅Prunus mume(BAE48658)、枇杷Eriobotryajaponica(ABV44810)和葡萄Vitis vinifera(CAO21890)的相似性分别达到97.8%、95%、92.6%和83.4%。
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| 关 键 词： | 梨 肉桂醇脱氢酶 RT-PCR |
Cloning of Cinnamyl Alcohol Dehydrogenase Genes from Pear Fruit |
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| Tao Shutian
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Zhao Mei
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Zhang Biao
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Khanizadeh Shahrokh
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Zhang Shaoling.Cloning of Cinnamyl Alcohol Dehydrogenase Genes from Pear Fruit[J].South China Fruits,2012,41(3):40-45. |
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| Authors: | Tao Shutian Zhao Mei Zhang Biao Khanizadeh Shahrokh Zhang Shaoling |
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| Institution: | 1(1Pear Engineering Research Center,Nanjing Agricultural University,Nanjing,210095;2 Horticultural Research and Development Centre,Agriculture and Agri-Food Canada) |
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| Abstract: | Cinnamyl alcohol dehydrogenase(CAD) plays a key role in lignin biosynthesis.Two cDNA fragments,designated as PB-CAD(GenBank accession No.FJ478151) and PP-CAD(GenBank accession No.FJ478152),coding for CAD were cloned using RT-PCR from ’Dangshansuli’ and ’Kousui’ pear cultivars and sequenced,respectively.Both cDNAs were 689 bp in length,and differed by only 4 bp in nucleotide sequences.Theoretically,they would encode two peptides of 229 amino-acid residues that shared 99.6% of identity with each other.Sequence alignment showed that the two deduced amino acid sequences were 97.8% identical to CAD from Malus domestica(AAC06319),95% to CAD from Prunus mume(BAE48658),92.6% to CAD from Eriobotrya japonica(ABV44810)and 83.4% to CAD from Vitis vinifera(CAO21890),respectively. |
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| Keywords: | pear(Pyrus) cinnamyl alcohol dehydrogenase RT-PCR |
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