| 内毒素对肝脏p53表达及Ca^2＋浓度的影响 |
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| 引用本文： | 李勇,高洪,严玉霖,杨桂梅,孔令青,刘海峰,彭洁.内毒素对肝脏p53表达及Ca^2＋浓度的影响[J].兽医大学学报,2012(6):881-883,888. |
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| 作者姓名： | 李勇 高洪 严玉霖 杨桂梅 孔令青 刘海峰 彭洁 |
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| 作者单位： | [1]云南农业大学动物科学技术学院,云南昆明650201 [2]云南省药物研究所GLP中心,云南昆明650111 [3]昆明医学院,云南昆明650031 [4]云南农业职业技术学院,云南昆明650212 |
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| 基金项目： | 国家自然科学基金资助项目（30560113）;云南省高端科技人才引进计划项目（2009C1125）;云南省现代农业生猪产业技术体系建设项目（云财农[2009]171号） |
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| 摘 要： | 为探讨内毒素在致大鼠肝损伤过程中对肝脏p53表达及ca^2＋浓度的影响，将48只SD大鼠随机分2组：Ⅰ组为NS对照组；Ⅱ组为ET致病组，2组大鼠分别于第3、4、8、12h每组各处死6只，采集肝脏分别用于p53表达的Western-blotting检测和ca^2＋浓度的FCM检测。结果显示，Ⅱ组p53的表达明显高于Ⅰ组（P＜0．01），且Ⅱ组一直呈上升趋势；Ⅱ组Ca^2＋的荧光指数明显高于Ⅰ组（P＜0．01），且一直呈上升趋势。结果表明，ET能有效上调肝细胞p53蛋白的表达及Ca^2＋浓度。
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| 关 键 词： | 内毒素 肝脏 p53蛋白 Ca^2＋浓度 |
Effect of endotoxin on expression of p53 and concentration of Ca^2＋ in liver of rats |
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| Authors: | LI Yong GAO Hong YAN Yu-lin YANG Gui-mei KONG Ling-qing LIU Hai-feng PENGJie |
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| Institution: | 1. College of Animal Science and Technology, Yunnan Agriculture University, Kunming650201 ,China; 2. Yunnan Institute of Materia Medica , GLP Centre, Kunming 650111, China; 3.Kunming Medical University ,Kunming 650031, China;4. Yunnan Agricultural Occupation Tech-nical College ,Kunming 650212, China) |
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| Abstract: | To investigate the effect of endotoxin on expression of p53 and concentration of Ca2＋ in the damaged liver of rats induced by ET. Forty-eight Sprague-Dawley rats were randomly divided into two groups., the normal saline controls group（group Ⅰ）and ET treatment group（group Ⅱ）. at 3,4,8 and 12 h past-treatment liver samples were taken for testing the expression of p53 by Western-blotting and the concentration of Ca^2＋ by FCM. For the expres- sion of p53 and the fluorescence index （FI） of Ca^2＋ ,groupⅡ was markedly higher than those of group Ⅰ （P〈0.01） with a rising trend. The results indicated that ET could up-regulate the p53 protein expression and the concentration of Ca^2＋ of hepatocyte in rats. |
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| Keywords: | endotoxin liver p53 protein concentration of Ca^2＋ |
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